User:Isis Trenchard/Notebook/BioE44 Stuff/2010/03/19: Difference between revisions
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*#safety stations - eye wash, shower | *#safety stations - eye wash, shower | ||
*# stuff to be careful about - EtBr, using transilluminator | *# stuff to be careful about - EtBr, using transilluminator | ||
==2nd day outline== | |||
*quiz over whatever reading was assigned | |||
**figure out what they should read - something about transformations, plasmids, antibiotic resistance.... making electrocompetant cells | |||
**tell them beforehand that theyll be getting a plasmid so they can start figuring out what to do with it.. how to figure out what it is doing. | |||
*everybody gets a plasmid - maybe measure concentration and make a dilution? because only need a tiny amount of plasmid for transformation.. | |||
*Make electrocompetent cells | |||
*transform plasmid in by electroporation | |||
*plate on amp and kan plates plus controls | |||
==3rd day== | |||
*have the come in the night before to inoculate cultures to do minipreps on tuesday. | |||
*quiz? | |||
*miniprep | |||
*digestion | |||
*have vectors prepped already | |||
*ligation | |||
*transformation (make e-comp cells again..) | |||
==Inventory== | ==Inventory== |
Revision as of 14:31, 19 March 2010
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Making electrocompetent cells protocol
First day of class outlineadapted from MIT 20.109
2nd day outline
3rd day
InventoryAt each bench (2 people per bench):
At each bay (for 2 pairs of people):
Stuff that needs to be made/done for the first weekFor the first day:
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