User:Isis Trenchard/Notebook/BioE44 Stuff/2010/03/19: Difference between revisions
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==First day of class outline== | ==First day of class outline== | ||
''adapted from [[20.109(S07):Lab_tour | MIT 20.109]]'' | |||
*'''Pipetting''' (micropipettes and pipetaids) - guided | |||
*#Make dilutions into cuvettes | |||
*'''Making Solutions''' (pH meter) - self guided | |||
*'''Spectrophotometer''' (and nanodrop) - self guided | |||
*'''Sterile Technique''' - guided | |||
*#Setting up your work area | |||
*#Maintaining a clean environment - EtOH bench, pipets | |||
*#Working with sterile solutions - using flame | |||
*#Aspirating | |||
*'''Lab Math''' (dilutions etc)- self guided | |||
*'''Lab Safety''' - self guided | |||
*#waste disposal - biohazard, glass, chemical | |||
*#safety stations - eye wash, shower | |||
*# stuff to be careful about - EtBr, using transilluminator | |||
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Revision as of 13:06, 19 March 2010
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Making electrocompetent cells protocol
First day of class outlineadapted from MIT 20.109
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