User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/08/25: Difference between revisions

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==U937 Delta ST on VCAM-1 and Poly-L-lysine, activated (N-fMLFF)==
==Jurkat E6-1 on VCAM-1==


'''Sample 1:''' Coverslips base-treated, coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30  minutes at room temperature, and gently washed 2 times with Hepes buffer. Treated with 0.5g% BSA for 30 minutes. Cells resuspended in Hepes on the coverslips for 30 minutes at room temperature, then washed with Hepes to remove unattached cells. Finally, treated with N-fMLFF <math>10^{-7}</math> PBS buffer.
Coverslips base-treated, coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30  minutes at room temperature, and gently washed 2 times with Hepes buffer. Cells plated in growth media on the coverslips for 30 minutes at room temperature.


[[Image:Jk082510-1.jpg]]
[[Image:Jk082510-1.jpg]]


Cells are attached, but remain globular with no protrusions. Maximum cell diameter in XY plane is seen at about 2 <math>\mu m</math> height. No significant cytoplasmic activity.  
Cells develop nice uniformly thin lamella.




Revision as of 17:28, 25 August 2010

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Jurkat E6-1 on VCAM-1

Coverslips base-treated, coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30 minutes at room temperature, and gently washed 2 times with Hepes buffer. Cells plated in growth media on the coverslips for 30 minutes at room temperature.

Cells develop nice uniformly thin lamella.



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