User:Igor R. Kuznetsov/Notebook/RBL-2H3/2010/08/25: Difference between revisions
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== | ==U937 Delta ST on VCAM-1 and Poly-L-lysine, activated (N-fMLFF)== | ||
'''Sample 1:''' Coverslips base-treated, coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30 minutes at room temperature, and gently washed 2 times with Hepes buffer. Treated with 0.5g% BSA for 30 minutes. Cells resuspended in Hepes on the coverslips for 30 minutes at room temperature, then washed with Hepes to remove unattached cells. Finally, treated with N-fMLFF <math>10^{-7}</math> PBS buffer. | |||
[[Image:080310_01.jpg]] | |||
Cells are attached, but remain globular with no protrusions. Maximum cell diameter in XY plane is seen at about 2 <math>\mu m</math> height. No significant cytoplasmic activity. | |||
Revision as of 17:25, 25 August 2010
 RBL-2H3
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U937 Delta ST on VCAM-1 and Poly-L-lysine, activated (N-fMLFF)Sample 1: Coverslips base-treated, coated with Recombinant Human VCAM-1/CD106 Fc Chimera (R&D Systems #P19320) for 30 minutes at room temperature, and gently washed 2 times with Hepes buffer. Treated with 0.5g% BSA for 30 minutes. Cells resuspended in Hepes on the coverslips for 30 minutes at room temperature, then washed with Hepes to remove unattached cells. Finally, treated with N-fMLFF [math]\displaystyle{ 10^{-7} }[/math] PBS buffer.
Cells are attached, but remain globular with no protrusions. Maximum cell diameter in XY plane is seen at about 2 [math]\displaystyle{ \mu m }[/math] height. No significant cytoplasmic activity.
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