User:Helen L. Slucher/Notebook/CHEM 571/2013/10/22: Difference between revisions

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### 210
### 210
## Water up to 5mL
## Water up to 5mL
==Notes==
*Stock Solutions
# HRP: 15.3 μM
# Pepsin: 15.7 μM
# Gold: 2.52 mM


==Data==
==Data==
Stock Solutions
*[[Image:Screen shot 2013-10-27 at 9.40.12 PM.png]]
# HRP
*[[Image:Screen shot 2013-10-27 at 9.40.37 PM.png]]
## 67.3mg in 100mL of water ---> 15.3 μM
*[[Image:Screen shot 2013-10-27 at 9.40.47 PM.png]]
# Pepsin
*[[Image:Screen shot 2013-10-27 at 9.40.55 PM.png]]
## 54.4mg in 100mL of water ---> 15.7 μM
*[[Image:Screen shot 2013-10-27 at 9.42.23 PM.png]]
# Gold
## Need 50mL of 2.5mM
## 49.7mg of HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water --> 2.52 mM
 


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Revision as of 18:45, 27 October 2013

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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Description

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Notes

  • Stock Solutions
  1. HRP: 15.3 μM
  2. Pepsin: 15.7 μM
  3. Gold: 2.52 mM

Data


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