User:Helen L. Slucher/Notebook/CHEM-572/2014/04/01: Difference between revisions

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==Goals==
==Goals==
# Test UV/Vis of pellet from GNP spin down
# Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
** Check to see if GNPs are there and look at size
# Spin down gamma method HRP pellet
# Spin down protein GNPs
# Remake solutions so they are more concentrated
** Take supernatant out and resuspend pellet
 
==Notes: Kinetics==
Reduction of heme group
OPD, look for diamino...change of concentration over time, rates
Change in absorbance = change in concentration
Find molar absorptivity
Deoxygen/oxygen bound
Take off clear super, spin down until it doesn't show GNP activity




Revision as of 10:43, 1 April 2014

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Goals

  1. Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
  2. Spin down gamma method HRP pellet
  3. Remake solutions so they are more concentrated

Notes: Kinetics

Reduction of heme group OPD, look for diamino...change of concentration over time, rates Change in absorbance = change in concentration Find molar absorptivity Deoxygen/oxygen bound Take off clear super, spin down until it doesn't show GNP activity



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