User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29: Difference between revisions
From OpenWetWare
Line 13: | Line 13: | ||
*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth | *was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth | ||
*Made M9 solution: | *Made M9 solution: | ||
Per 1 L of M9: Adjusted for 700mL | Per 1 L of M9: Adjusted for 700mL Notes: | ||
-KH2PO4 3g 2.1g | -KH2PO4 3g 2.1g FisherChemicals F.W.141.96, lot no. 005502 Date:8.07.00 | ||
-Na2HPO4 6g 4.2g | -Na2HPO4 6g 4.2g FisherChemicals F.W. 136.09, lot no. 995072 Date? | ||
-NaCl 5g 3.5g | -NaCl 5g 3.5g NaCl F.W. 58.44 | ||
-H2O to 1L water to 700mL | -H2O to 1L water to 700mL MilliQ, prepared by Skyler | ||
-read below 700µL MgSO4 | -read below 700µL MgSO4 1 M prepared by Lena, aliquoted 29 May 2013 | ||
Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) | Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) |
Revision as of 16:12, 29 May 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
29 May 2013
-conc. from 10mL LB to 1.5mL -blinded for adam: L4440=squiggles, GFP=wiggles
Per 1 L of M9: Adjusted for 700mL Notes: -KH2PO4 3g 2.1g FisherChemicals F.W.141.96, lot no. 005502 Date:8.07.00 -Na2HPO4 6g 4.2g FisherChemicals F.W. 136.09, lot no. 995072 Date? -NaCl 5g 3.5g NaCl F.W. 58.44 -H2O to 1L water to 700mL MilliQ, prepared by Skyler -read below 700µL MgSO4 1 M prepared by Lena, aliquoted 29 May 2013 Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave) add 1mL of MgSO4 sterily
|