User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/17: Difference between revisions
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== | ==17 May 2013== | ||
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Lab Meeting - see [https://docs.google.com/a/alaska.edu/document/d/1WEjKBAGd4NUJLZjGYykSZDrkruUn0_0Nxk0Gc3k3cMU/edit?usp=sharing Memos - Senile Worms] | |||
Chunk ID1:B21 worms to new plate | |||
-plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon! | |||
ROS assay with Courtney and Skyler | |||
-transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms | |||
-washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces | |||
-moved samples onto 96 well plate in sections F1-3 to H1-3 | |||
-used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye | |||
RESULTS | |||
Inoculated OP50 for seeding plates | |||
-10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013 | |||
-8 ml LB w/ 4µl strep. for stock plates | |||
Revaluated Schedule for RNAi treatments [https://docs.google.com/a/alaska.edu/spreadsheet/ccc?key=0At5kTTSjupzTdHhuMldJbEQtMWpBSlpTSXh0aGw4TGc&usp=sharing RNAi Screen Schedule] | |||
Revision as of 15:11, 17 May 2013
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17 May 2013Lab Meeting - see Memos - Senile Worms Chunk ID1:B21 worms to new plate -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon! ROS assay with Courtney and Skyler -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms
-washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces
-moved samples onto 96 well plate in sections F1-3 to H1-3
-used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
RESULTS
Inoculated OP50 for seeding plates -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
-8 ml LB w/ 4µl strep. for stock plates
Revaluated Schedule for RNAi treatments RNAi Screen Schedule
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