User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==17 May 2013==
* Insert content here...
 
*Lab Meeting - see [https://docs.google.com/a/alaska.edu/document/d/1WEjKBAGd4NUJLZjGYykSZDrkruUn0_0Nxk0Gc3k3cMU/edit?usp=sharing Memos - Senile Worms]
*Chunk ID1:B21 worms to new plate
      -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon!
*ROS assay with Courtney and Skyler
      -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms
      -washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces
      -moved samples onto 96 well plate in sections F1-3 to H1-3
      -used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
      RESULTS
*Inoculated OP50 for seeding plates
      -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
      -8 ml LB w/ 4µl strep. for stock plates
 
*Revaluated Schedule for RNAi treatments [https://docs.google.com/a/alaska.edu/spreadsheet/ccc?key=0At5kTTSjupzTdHhuMldJbEQtMWpBSlpTSXh0aGw4TGc&usp=sharing RNAi Screen Schedule]
 




Latest revision as of 22:42, 26 September 2017

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17 May 2013

     -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon!
  • ROS assay with Courtney and Skyler
     -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms 
     -washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces 
     -moved samples onto 96 well plate in sections F1-3 to H1-3
     -used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
     RESULTS
  • Inoculated OP50 for seeding plates
     -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
     -8 ml LB w/ 4µl strep. for stock plates



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