User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/08: Difference between revisions
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==Objectives== | ==Objectives== | ||
#Run UV-vis for | #Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found below under Data. | ||
==Description== | ==Description== | ||
#Test solutions made according to below concentrations under Data. | #Test solutions made according to below concentrations under Data. | ||
#On 11/02, solutions were placed into the oven at 80°C for 4 hours. | #On 11/02, solutions were placed into the oven at 80°C for 4 hours. | ||
#UV-vis data was run on each of the | #UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations. | ||
#After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly. | #After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly. | ||
==Data== | ==Data== | ||
'''Solution concentrations'''<br> | '''Solution concentrations'''<br> | ||
<u>UV-vis solutions for 11. | <u>UV-vis solutions for 11.02.2016</u><br> | ||
[[Image:Screen Shot 2016-11- | [[Image:Screen Shot 2016-11-15 at 11.07.53 PM.png]]<br> | ||
[[Image: | [[Image:102616 Abs Wave Fruc.75.png|thumb|upright=4.5]] <br> | ||
'''Figure 1''' Absorbance v wavelength of lysozyme in 0. | '''Figure 1''' Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose | ||
[[Image: | [[Image:102616 Abs 279 Fruc.75.png|thumb|upright=4.5]] <br> | ||
'''Figure 2''' Absorbance at | '''Figure 2''' Absorbance at 279 nm at various pH's in 0.75 mM concentration of fructose | ||
[[Image: | [[Image:102616 Abs Wave Fruc1.25.png|thumb|upright=4.5]] <br> | ||
'''Figure 3''' Absorbance v wavelength of lysozyme in | '''Figure 3''' Absorbance v wavelength of lysozyme in 1.25 mM concentration of fructose | ||
[[Image: | [[Image:102616 Abs 279 Fruc1.25.png|thumb|upright=4.5]] <br> | ||
'''Figure 4''' Absorbance at 279 nm at various pH's in 1.25 mM concentration of fructose <br> | |||
'''Figure | |||
==Notes== | ==Notes== | ||
All Figures in the above data are baseline and water corrected. <br> | All Figures in the above data are baseline and water corrected. <br> | ||
After taking the UV-vis samples out of the 80°C oven, pH 11 for both Fructose concentrations showed a precipitate form. pH 12 did not show this precipitate. | |||
Revision as of 12:06, 30 November 2016
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Objectives
Description
DataSolution concentrations  Figure 1 Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose  Figure 2 Absorbance at 279 nm at various pH's in 0.75 mM concentration of fructose  Figure 3 Absorbance v wavelength of lysozyme in 1.25 mM concentration of fructose  Figure 4 Absorbance at 279 nm at various pH's in 1.25 mM concentration of fructose NotesAll Figures in the above data are baseline and water corrected.
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