User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/25: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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'''Figure 1''' Maximum emission data in a solution of pH 9.<br>
'''Figure 1''' Maximum emission data in a solution of pH 9.<br>


[[Image:100416 Int v Time Lys pH 7.png|thumb|upright=5.0]]<br>
[[Image:102516 Int Time Lys pH9.png|thumb|upright=5.0]]<br>
'''Figure 2''' Integrated fluorescence data in a solution of pH . <br>
'''Figure 2''' Integrated fluorescence data in a solution of pH 7. <br>


[[Image:100416 Full Fluor Lys pH 7.png|thumb|upright=5.0]]<br>
[[Image:102516 Fluor pH9.png|thumb|upright=5.0]]<br>
'''Figure 3''' Full emission spectra for important time points: t=0, 15, 33, 105, 180 min. <br>
'''Figure 3''' Full emission spectra for important time points: t=0, 6, 33, 90, 180 min. <br>


<u>'''Figure 3''' Explanation</u><br>
<u>'''Figure 3''' Explanation</u><br>
#At t=0 min, we see a max emission far to the left of the other emission spectra.  
#At t=0 min, we see a max emission far to the left of the other emission spectra.  
#At t=15 min, this maximum has shifted up and to the right.  
#At t=6 min, this maximum has shifted up and to the right.  
#Until t=33 min, the maximum for each spectra decreases until the shown maximum.  
#Until t=33 min, the maximum for each spectra decreases until the shown maximum.  
#After t=33 min, the maximum begins increasing again.  
#After t=33 min, the maximum begins increasing again.  
#At t=105 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.  
#At t=90 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.  
#t=180 shows the final full spectra obtained.<br>
#t=180 shows the final full spectra obtained.<br>


<u>UV-vis solutions for 09.14.2016</u><br>
<u>UV-vis solutions for 10.26..2016</u><br>
[[Image:102616 Fruc .75mM.png]]<br>
[[Image:102616 Fruc .75mM.png]]<br>
'''Table 1''' UV-vis solutions made for [Fructose]=.75mM with silver  
'''Table 1''' UV-vis solutions made for [Fructose]=.75mM with silver  


[[Image:Screen Shot 2016-10-25 at 10.08.51 PM.png]<br>
[[Image:Screen Shot 2016-11-15 at 2.10.46 PM.png]]<br>
'''Table 2''' UV-vis solutions made for [Fructose]=1.25mM with silver
'''Table 2''' UV-vis solutions made for [Fructose]=1.25mM with silver


Latest revision as of 02:07, 27 September 2017

Project name Main project page
Previous entry      Next entry

Objectives

  1. Run fluorescence data for lysozyme at pH=9
  2. Create stock solutions for use on 10.26.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 10/05/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data


Figure 1 Maximum emission data in a solution of pH 9.


Figure 2 Integrated fluorescence data in a solution of pH 7.


Figure 3 Full emission spectra for important time points: t=0, 6, 33, 90, 180 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=6 min, this maximum has shifted up and to the right.
  3. Until t=33 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=33 min, the maximum begins increasing again.
  5. At t=90 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=180 shows the final full spectra obtained.

UV-vis solutions for 10.26..2016

Table 1 UV-vis solutions made for [Fructose]=.75mM with silver


Table 2 UV-vis solutions made for [Fructose]=1.25mM with silver



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