User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/12: Difference between revisions

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==Entry title==
==Objectives==
* Insert content here...
#Run fluorescence data for lysozyme at pH=8
#Create stock solutions for use on 10.18.2016


==Description==
<u>Fluorescence</u>
#Solutions made using measurements below
#Fluorescence data run every 3 minutes for 3 hours
<u>Solutions for 10/18/2016</u><br>
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.
==Data==
[[Image:101216 Lys Emis v time pH 8.png|thumb|upright=5.0]]<br>
'''Figure 1''' Maximum emission data in a solution of pH 8.<br>
[[Image:101216 Lys Int v time pH 8.png|thumb|upright=5.0]]<br>
'''Figure 2''' Integrated fluorescence data in a solution of pH 8. <br>
[[Image:101216 Lys Fluor Summary pH 8.png|thumb|upright=5.0]]<br>
'''Figure 3''' Full emission spectra for important time points: t=0, 3, 21, 39, 159, 180 min. <br>
<u>'''Figure 3''' Explanation</u><br>
#At t=0 min, we see a max emission far to the left of the other emission spectra.
#At t=3 min, this maximum has shifted up and to the right.
#Until t=39 min, the maximum for each spectra decreases until the shown maximum.
#After t=39 min, the maximum begins increasing again.
#At t=159 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
#t=180 shows the final full spectra obtained.<br>
<u>UV-vis solutions for 10.18.2016</u><br>
[[Image:101816 UVvis Conc Fruc .125mM.png]]<br>
'''Table 1''' UV-vis solutions made for [Fructose]=.125mM with silver
[[Image:101816 UVvis Conc Fruc .25mM.png]]<br>
'''Table 2''' UV-vis solutions made for [Fructose]=.25mM with silver


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Revision as of 11:54, 25 October 2016

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Objectives

  1. Run fluorescence data for lysozyme at pH=8
  2. Create stock solutions for use on 10.18.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 10/18/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data


Figure 1 Maximum emission data in a solution of pH 8.


Figure 2 Integrated fluorescence data in a solution of pH 8.


Figure 3 Full emission spectra for important time points: t=0, 3, 21, 39, 159, 180 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=3 min, this maximum has shifted up and to the right.
  3. Until t=39 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=39 min, the maximum begins increasing again.
  5. At t=159 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=180 shows the final full spectra obtained.

UV-vis solutions for 10.18.2016

Table 1 UV-vis solutions made for [Fructose]=.125mM with silver


Table 2 UV-vis solutions made for [Fructose]=.25mM with silver


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