User:Guido/Notes/July 07: Difference between revisions
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m (July 07 moved to User:Guido/Notes/July 07) |
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| Line 6: | Line 6: | ||
** 3 μl culture | ** 3 μl culture | ||
** 1 μl f-primer | ** 1 μl f-primer | ||
** 1 μl r-primer | ** 1 μl r-primer | ||
** | ** 45 μl supermix | ||
** 50 μl reaction | ** 50 μl reaction | ||
* | * 95 10 min | ||
* | * 95 30 sec | ||
* | * 51 30 sec | ||
* 72 2 min | * 72 2 min | ||
* X30 | |||
* 72 7 min | |||
* 72 | |||
* 4 forever | * 4 forever | ||
Latest revision as of 08:35, 3 July 2007
July 3, 2007
- Do a culture pcr from last recombination of specR to replace MutS
- 3 μl culture
- 1 μl f-primer
- 1 μl r-primer
- 45 μl supermix
- 50 μl reaction
* 95 10 min * 95 30 sec * 51 30 sec * 72 2 min * X30 * 72 7 min * 4 forever
