User:Etienne Robillard/Notebook/HEK293

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Human Embryonic Kidney cells  
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== HEK293: Functionalizing abortion and human trans-splicing experiments via Human Embryonic Kidney 293 cells by Etienne Robillard ==
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* http://webserver.mbi.ufl.edu/~shaw/293.html
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__TOC__
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Tasting Human Embryonic Kidney Cells In Your Synthetic Gastronomy: The Evil is in the details
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=== History of HEK 293  ===
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* 1977: Frank Graham et al (Alex Van der Eb) reported the use HEK293 as a Calcium phosphate transfection reagent.
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** <i>"HEK 293 cells were generated by transformation of human embryonic kidney cell cultures (hence HEK) with sheared adenovirus 5 DNA, and were first described in 1977 (Graham et al., J. Gen Virol 1977 Jul;36(1):59-74). The story of this cell line is quite an interesting one, getting to the very roots of modern molecular biology. Frank Graham was working with Alex Van der Eb in the University of Leiden in Holland, and developed the calcium phosphate method of transfecting eukaryotic cells, an astonishing technique described in a classic paper (Graham and Van der Eb. Virology 1973 Apr;52(2):456-67); <font color="purple" style="background-color:#ddd"><b>who would have thought that you would only have to mix DNA with a bit of calcium phosphate to get the DNA incorporated and even expressed in mammalian cells?</b></font> The story of this discovery was described by Graham himself for Current Contents in 1988 (link to *.pdf here). By 1977 Graham had moved to Canada, and from there produced the other classical paper describing the derivation of the HEK 293 cells. There is also an interesting Current Contents article by Graham describing this work (link to *.pdf here).</i> [1]
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* 2001: FDA decides to approve HEK293 deviation as an experimental transfection reagent for neuronal trans-splicing of mammalian DNA into the human genome.
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** <i>Also, check out the transcript of an FDA meeting entitled "Vaccines and related biological products advisory committee" which took place in May 2001. At this meeting Alex Van der Eb describes the work that led to the HEK 293 derivation. I was particularly fascinated that he actually speculated that these cells may have originated from a rare neuronal cell in the kidney cell cultures, since at about the same time but completely independently we had come to a similar conclusion (link to *.pdf here - Dr. Van der Eb's part starts on page 77, and the specific comment about the potential neuronal origin of 293 cells is on page 85).</i>"[1]
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* 2005: MIT scientists developed an experimental [http://openwetware.org/wiki/Escherichia_coli/Methylation DNA methylation] technique for inter-species, mutant protein acquisition and over-expression of the '''PRMT5''' gene into the human genome. [3]
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* 2006: Schmitt et al (US) crafted a new assay for cross-species methylation of the Wnt3 gene into mammalian hosts. [4] [[doi:10.1038/nature04334]]
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=== Glossary ===
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* Human
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* Embryo
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* Embryonic
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* Kidney
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* Cells
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* Placenta [2]
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* Trans splicing
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* mRNA
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* Neuron
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* DNA
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* Chick optic tectum
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=== References ===
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# http://webserver.mbi.ufl.edu/~shaw/293.html  
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# http://embryology.med.unsw.edu.au/Notes/placenta5.htm
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# http://openwetware.org/wiki/Silver:_Methylation_Assay
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# http://openwetware.org/wiki/Bio154JM08/Toolbox/Paper_1#HEK293_cells
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=== See also ===
* http://gerardnadal.com/2011/06/01/pepsi-aborted-babies-ethics-and-tasteful-research/
* http://gerardnadal.com/2011/06/01/pepsi-aborted-babies-ethics-and-tasteful-research/
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* TRPV1 gene protein (transient receptor) expression : http://www.faqs.org/patents/app/20090221511
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** "Abstract: A process is disclosed for controlling inflammatory tissue access through release of neuropeptides, such as substance P (sP), to insulin-responsive sensory neurons, whereby simultaneous control of insulin sensitivity/resistance is manifested. In models of Type 1 and Type 2 diabetes, '''sensory''' afferents, '''in particular TRPV1, have fundamental roles in insulin/glucose homeostasis, islet physiology and autoimmune tissue inflammation.'''"  
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=== Did you know? ===
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* keywords: TRPV1, [http://student.biology.arizona.edu/honors98/group12/pepper.html capsaicin], [http://en.wikipedia.org/wiki/Substance_P Substance P (SP)], neuropeptide, transient sensory receptor, key transient ligand transmitter, antidepressant, 5-HT agonist (serotonin), #hek293, #abortion, #taste, #Ethics, #NMDAR :U
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"Chuck Norris can model the behaviour of an oscillator without any parameters." [http://2010.igem.org/Team:Slovenia/PROJECT/proof/popfret/fret Link]

Current revision

HEK293: Functionalizing abortion and human trans-splicing experiments via Human Embryonic Kidney 293 cells by Etienne Robillard

Contents


Tasting Human Embryonic Kidney Cells In Your Synthetic Gastronomy: The Evil is in the details

History of HEK 293

  • 1977: Frank Graham et al (Alex Van der Eb) reported the use HEK293 as a Calcium phosphate transfection reagent.
    • "HEK 293 cells were generated by transformation of human embryonic kidney cell cultures (hence HEK) with sheared adenovirus 5 DNA, and were first described in 1977 (Graham et al., J. Gen Virol 1977 Jul;36(1):59-74). The story of this cell line is quite an interesting one, getting to the very roots of modern molecular biology. Frank Graham was working with Alex Van der Eb in the University of Leiden in Holland, and developed the calcium phosphate method of transfecting eukaryotic cells, an astonishing technique described in a classic paper (Graham and Van der Eb. Virology 1973 Apr;52(2):456-67); who would have thought that you would only have to mix DNA with a bit of calcium phosphate to get the DNA incorporated and even expressed in mammalian cells? The story of this discovery was described by Graham himself for Current Contents in 1988 (link to *.pdf here). By 1977 Graham had moved to Canada, and from there produced the other classical paper describing the derivation of the HEK 293 cells. There is also an interesting Current Contents article by Graham describing this work (link to *.pdf here). [1]
  • 2001: FDA decides to approve HEK293 deviation as an experimental transfection reagent for neuronal trans-splicing of mammalian DNA into the human genome.
    • Also, check out the transcript of an FDA meeting entitled "Vaccines and related biological products advisory committee" which took place in May 2001. At this meeting Alex Van der Eb describes the work that led to the HEK 293 derivation. I was particularly fascinated that he actually speculated that these cells may have originated from a rare neuronal cell in the kidney cell cultures, since at about the same time but completely independently we had come to a similar conclusion (link to *.pdf here - Dr. Van der Eb's part starts on page 77, and the specific comment about the potential neuronal origin of 293 cells is on page 85)."[1]
  • 2005: MIT scientists developed an experimental DNA methylation technique for inter-species, mutant protein acquisition and over-expression of the PRMT5 gene into the human genome. [3]
  • 2006: Schmitt et al (US) crafted a new assay for cross-species methylation of the Wnt3 gene into mammalian hosts. [4] doi:10.1038/nature04334

Glossary

  • Human
  • Embryo
  • Embryonic
  • Kidney
  • Cells
  • Placenta [2]
  • Trans splicing
  • mRNA
  • Neuron
  • DNA
  • Chick optic tectum

References

  1. http://webserver.mbi.ufl.edu/~shaw/293.html
  2. http://embryology.med.unsw.edu.au/Notes/placenta5.htm
  3. http://openwetware.org/wiki/Silver:_Methylation_Assay
  4. http://openwetware.org/wiki/Bio154JM08/Toolbox/Paper_1#HEK293_cells

See also

Did you know?

"Chuck Norris can model the behaviour of an oscillator without any parameters." Link

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