User:Etienne Robillard/Notebook/Chemtrails911 notebook/ForensicEvidencesChart

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(Input/output promoters)
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(References)
 
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== Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Chemtrails ==
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== Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Agent E.coli ==
'''"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)."''' [http://www.trdd.org/EUGBR_2E.HTM#_Toc511642187 1]
'''"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)."''' [http://www.trdd.org/EUGBR_2E.HTM#_Toc511642187 1]
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=== Synopsis ===
=== Synopsis ===
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The goal of this project is to collect scientific evidences of state-sponsored collusion in the research and development of a precise technology known
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What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional
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as a '''"genetic switch"''', or "transcriptional devices", ie a well-designed biological system to induce early genetic mutations and organ failures in humans on the premise of scientific advancement
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conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate
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and eugenics.
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and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?
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The aim of this page is to collect data on so-called '''genetic switches''', or synthetic '''transcriptional devices''' and rationalize the evidences to justify the validity
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of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.
=== Evidence collection ===
=== Evidence collection ===
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|-
|-
! scope="col" | Promoter name  
! scope="col" | Promoter name  
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! scope="col" | Promoter type  
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! scope="col" | Promoter type
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! scope="col" | Description
! scope="col" | Target organisms
! scope="col" | Target organisms
! scope="col" | Restriction site   
! scope="col" | Restriction site   
! scope="col" | EC number  
! scope="col" | EC number  
! scope="col" | References
! scope="col" | References
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! scope="col" | Biobrick Part
|-
|-
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! scope="row" | Arabinose
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! scope="row" | L-arabinose
| aldopentose
| aldopentose
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| E.coli (K-12/MG1655), P.fluorescens, Humans
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| Inducible pBAD/araC promoter
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| E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens
| araBAD (''araC'')
| araBAD (''araC'')
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| 1.1.1.46
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|  
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| [http://openwetware.org/wiki/Arabinose 1], [http://en.wikipedia.org/wiki/Arabinose 2], [http://metacyc.org/ECOLI/NEW-IMAGE?type=NIL&object=TU00214&redirect=T 3]
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| [http://openwetware.org/wiki/Arabinose 1], [http://en.wikipedia.org/wiki/Arabinose 2], [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6976.2010.00226.x/abstract 3], [http://www.ncbi.nlm.nih.gov/pubmed/12080425?dopt=Abstract 4], [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/regulation/AraC.html 5]
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| BBa_I0500
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|-
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! scope="row" | cAMP
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| second messenger
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| cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter.
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| Homo sapiens
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| LacZ
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| 3.1.4.17, 3.1.4.53, 4.6.1.1
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| [http://en.wikipedia.org/wiki/Cyclic_adenosine_monophosphate 1]
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|   
|}
|}
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==== Synthetic transcriptional devices ====
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==== Plasmid addiction systems ====
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[data here...]
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{| border="1" cellpadding="2" class="wikitable"
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|-
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! scope="col" | Plasmid name
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! scope="col" | Origin of replication
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! scope="col" | Organism (Strain)
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! scope="col" | Anti-bacterial resistance
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! scope="col" | Quorum sensing protein expression 
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! scope="col" | ColE1 (Colicins) protein expression
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! scope="col" | 6-'''his'''-tagged promoter
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! scope="col" | References
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|-
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| scope="row" | pSB1A[C]3 (formerly pSB1A2?)
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| scope="row" | pUC19-derived (pMB1)
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| scope="row" | ''E.coli'' ([http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783])
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| scope="row" | ampicilin (ampR)
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| scope="row" | Yes (GFP)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| scope="row" | [http://partsregistry.org/Part:pSB1A2 1], [http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP 2], [http://partsregistry.org/Part:BBa_I746914 3], [http://partsregistry.org/Part:BBa_I0500 4], [http://partsregistry.org/wiki/index.php?title=Part:BBa_P1010 5]
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|-
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| scope="row" | pSB3K3-1010
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| scope="row" | p15a-derived (pMR101)
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| scope="row" | ''E.coli'' (MG1655)
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| scope="row" | kanamycin (kanR)
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| scope="row" | Yes (GFP/RFP via F2620)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| [http://partsregistry.org/cgi/partsdb/related.cgi?part=F2620 1], [http://partsregistry.org/Part:BBa_F2620 2], [http://partsregistry.org/Part:BBa_V1000:Design 3]
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|}
==== Reagents ====
==== Reagents ====
[data here...]
[data here...]
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==== Discussion ====
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*  A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- [[User:Etienne Robillard|Etienne Robillard]] 21:45, 15 October 2012 (EDT)
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* Whether the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681472/ T7.1 bacteriophage] was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or [http://www.ncbi.nlm.nih.gov/pubmed/18410688 refactor]) DNA using a [http://www.ncbi.nlm.nih.gov/nuccore/169656098 F-plasmid like vector]. In bacterias, an equivalent process is known as [http://www.science-projects.com/F-sex.htm bacterial conjugation].
=== References ===
=== References ===
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<biblio>
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#Shetty-JBiolEng-2008 pmid=18410688
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#Atsumi-Little-PNAS-2006 pmid=17135356
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#Lutz-Bujard-Nucleic-Acid-Res-2007 pmid=9092630
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#Morgan-Kiss-PNAS-2002 pmid=12032290
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#Khlebnikov-Microbiology-2001 pmid=11739756
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#KA-Datsenko-BL-Wanner-2000 pmid=10829079
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</biblio>
=== Keywords ===
=== Keywords ===
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing

Current revision

Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Agent E.coli

"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)." 1

Contents


Synopsis

What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?

The aim of this page is to collect data on so-called genetic switches, or synthetic transcriptional devices and rationalize the evidences to justify the validity of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.

Evidence collection

Input/output promoters

Promoter name Promoter type Description Target organisms Restriction site EC number References Biobrick Part
L-arabinose aldopentose Inducible pBAD/araC promoter E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens araBAD (araC) 1, 2, 3, 4, 5 BBa_I0500
cAMP second messenger cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter. Homo sapiens LacZ 3.1.4.17, 3.1.4.53, 4.6.1.1 1

Plasmid addiction systems

Plasmid name Origin of replication Organism (Strain) Anti-bacterial resistance Quorum sensing protein expression ColE1 (Colicins) protein expression 6-his-tagged promoter References
pSB1A[C]3 (formerly pSB1A2?) pUC19-derived (pMB1) E.coli (BW27783) ampicilin (ampR) Yes (GFP) Yes (ccdB?) Yes (L-arabinose inducible) 1, 2, 3, 4, 5
pSB3K3-1010 p15a-derived (pMR101) E.coli (MG1655) kanamycin (kanR) Yes (GFP/RFP via F2620) Yes (ccdB?) Yes (L-arabinose inducible) 1, 2, 3

Reagents

[data here...]

Discussion

  • A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- Etienne Robillard 21:45, 15 October 2012 (EDT)
  • Whether the T7.1 bacteriophage was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or refactor) DNA using a F-plasmid like vector. In bacterias, an equivalent process is known as bacterial conjugation.

References

  1. Shetty RP, Endy D, and Knight TF Jr. . pmid:18410688. PubMed HubMed [Shetty-JBiolEng-2008]
  2. Atsumi S and Little JW. . pmid:17135356. PubMed HubMed [Atsumi-Little-PNAS-2006]
  3. Lutz R and Bujard H. . pmid:9092630. PubMed HubMed [Lutz-Bujard-Nucleic-Acid-Res-2007]
  4. Morgan-Kiss RM, Wadler C, and Cronan JE Jr. . pmid:12032290. PubMed HubMed [Morgan-Kiss-PNAS-2002]
  5. Khlebnikov A, Datsenko KA, Skaug T, Wanner BL, and Keasling JD. . pmid:11739756. PubMed HubMed [Khlebnikov-Microbiology-2001]
  6. Datsenko KA and Wanner BL. . pmid:10829079. PubMed HubMed [KA-Datsenko-BL-Wanner-2000]
All Medline abstracts: PubMed HubMed

Keywords

synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, transcriptional inverter, reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing

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