User:Etienne Robillard/Notebook/chim trills notebook/ForensicEvidencesChart

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== Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Agent E.coli ==
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'''"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)."''' [http://www.trdd.org/EUGBR_2E.HTM#_Toc511642187 1]
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__TOC__
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=== Synopsis ===
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What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional
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conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate
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and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?
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The aim of this page is to collect data on so-called '''genetic switches''', or synthetic '''transcriptional devices''' and rationalize the evidences to justify the validity
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of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.
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=== Evidence collection ===
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==== Input/output promoters ====
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{| border="1" cellpadding="2" class="wikitable"
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|-
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! scope="col" | Promoter name
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! scope="col" | Promoter type
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! scope="col" | Description
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! scope="col" | Target organisms
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! scope="col" | Restriction site 
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! scope="col" | EC number
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! scope="col" | References
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! scope="col" | Biobrick Part
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! scope="row" | L-arabinose
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| aldopentose
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| Inducible pBAD/araC promoter
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| E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens
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| araBAD (''araC'')
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| [http://openwetware.org/wiki/Arabinose 1], [http://en.wikipedia.org/wiki/Arabinose 2], [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6976.2010.00226.x/abstract 3], [http://www.ncbi.nlm.nih.gov/pubmed/12080425?dopt=Abstract 4], [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/regulation/AraC.html 5]
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| BBa_I0500
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|-
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! scope="row" | cAMP
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| second messenger
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| cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter.
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| Homo sapiens
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| LacZ
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| 3.1.4.17, 3.1.4.53, 4.6.1.1
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| [http://en.wikipedia.org/wiki/Cyclic_adenosine_monophosphate 1]
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|   
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|}
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==== Plasmid addiction systems ====
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{| border="1" cellpadding="2" class="wikitable"
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|-
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! scope="col" | Plasmid name
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! scope="col" | Origin of replication
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! scope="col" | Organism (Strain)
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! scope="col" | Anti-bacterial resistance
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! scope="col" | Quorum sensing protein expression 
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! scope="col" | ColE1 (Colicins) protein expression
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! scope="col" | 6-'''his'''-tagged promoter
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! scope="col" | References
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| scope="row" | pSB1A[C]3 (formerly pSB1A2?)
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| scope="row" | pUC19-derived (pMB1)
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| scope="row" | ''E.coli'' ([http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783])
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| scope="row" | ampicilin (ampR)
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| scope="row" | Yes (GFP)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| scope="row" | [http://partsregistry.org/Part:pSB1A2 1], [http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP 2], [http://partsregistry.org/Part:BBa_I746914 3], [http://partsregistry.org/Part:BBa_I0500 4], [http://partsregistry.org/wiki/index.php?title=Part:BBa_P1010 5]
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|-
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| scope="row" | pSB3K3-1010
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| scope="row" | p15a-derived (pMR101)
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| scope="row" | ''E.coli'' (MG1655)
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| scope="row" | kanamycin (kanR)
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| scope="row" | Yes (GFP/RFP via F2620)
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| scope="row" | Yes (ccdB?)
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| scope="row" | Yes (L-arabinose inducible)
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| [http://partsregistry.org/cgi/partsdb/related.cgi?part=F2620 1], [http://partsregistry.org/Part:BBa_F2620 2], [http://partsregistry.org/Part:BBa_V1000:Design 3]
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|}
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==== Reagents ====
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[data here...]
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==== Discussion ====
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*  A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- [[User:Etienne Robillard|Etienne Robillard]] 21:45, 15 October 2012 (EDT)
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* Whether the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681472/ T7.1 bacteriophage] was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or [http://www.ncbi.nlm.nih.gov/pubmed/18410688 refactor]) DNA using a [http://www.ncbi.nlm.nih.gov/nuccore/169656098 F-plasmid like vector]. In bacterias, an equivalent process is known as [http://www.science-projects.com/F-sex.htm bacterial conjugation].
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=== References ===
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<biblio>
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#Shetty-JBiolEng-2008 pmid=18410688
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#Atsumi-Little-PNAS-2006 pmid=17135356
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#Lutz-Bujard-Nucleic-Acid-Res-2007 pmid=9092630
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#Morgan-Kiss-PNAS-2002 pmid=12032290
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#Khlebnikov-Microbiology-2001 pmid=11739756
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#KA-Datsenko-BL-Wanner-2000 pmid=10829079
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</biblio>
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=== Keywords ===
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synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing
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