User:Etienne Robillard/Notebook/Chemtrails911 notebook/Agent Ecoli:Plasmid cloning vectors for gene therapy with phosphohistidine based oligos
From OpenWetWare
(Difference between revisions)
(→Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis) |
(→Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis) |
||
| Line 7: | Line 7: | ||
* N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent. | * N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent. | ||
* Reminder: | * Reminder: | ||
| - | ** NHC = (N heterocycle carbene) - Chemical compounds with a N chiral prodrug switch - highly enantioselective molecules | + | ** NHC = (N heterocycle carbene) - Chemical compounds with a N chiral prodrug switch - highly enantioselective molecules |
| + | ** N = indicates a Nitrogen atom | ||
| + | ** P = a Phosphonate (inorganic state) - a compound for oligo synthesis using the [http://en.wikipedia.org/wiki/Oligonucleotide_synthesis#Nucleoside_phosphoramidites nucleoside phosphoramidite] method. | ||
| + | ** P-N = phosphoramidate (a Nitrogen atom bound to a Phosphonate ester in nucleophilic substitution). | ||
== References == | == References == | ||
Revision as of 08:22, 6 November 2012
Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos
Contents |
Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis
cDNA:
- N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
- N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.
- Reminder:
- NHC = (N heterocycle carbene) - Chemical compounds with a N chiral prodrug switch - highly enantioselective molecules
- N = indicates a Nitrogen atom
- P = a Phosphonate (inorganic state) - a compound for oligo synthesis using the nucleoside phosphoramidite method.
- P-N = phosphoramidate (a Nitrogen atom bound to a Phosphonate ester in nucleophilic substitution).
