User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid cloning vectors for gene therapy with phosphohistidine based oligos

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(Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis)
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= Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos =
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{{deleteme}}
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__TOC__
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== Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis ==
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cDNA:
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* N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
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* N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.
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* Reminder:
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** NHC = (N heterocycle carbene) - Chemical compounds with a N chiral prodrug switch - highly enantioselective molecules
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** N = indicates a Nitrogen atom
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** P = a Phosphonate (inorganic state) - a compound for oligo synthesis using the [http://en.wikipedia.org/wiki/Oligonucleotide_synthesis#Nucleoside_phosphoramidites nucleoside phosphoramidite] method. 
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** P-N = phosphoramidate (a Nitrogen atom bound to a Phosphonate ester in nucleophilic substitution).
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deoxynucleotide synthesis:
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* cDNA synthesis building block = deoxynucleoside + 3'-phosphonate = synthetic nucleotide analog (dATP) [http://dwb4.unl.edu/Teacher/NSF/C08/C08Links/www.dur.ac.uk/~dbl0www/Staff/Croy/cDNAfigs.htm 1]
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oligodeoxyribonucleotide synthesis:
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* short 50-200(?) bp restriction endonucleases enzymes for cDNA catalysis/plasmid vector cloning in ''E. coli''
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* building blocks for oligonucleotides synthesis with phosphoramidites method [http://en.wikipedia.org/wiki/Oligonucleotide 1]
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* see also [http://openwetware.org/wiki/CH391L/S12/GeneandGenomeSynthesis#Oligonucleotide_Synthesis Oligonucleotide Synthesis]
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[http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering#Part_3:_Digest_M13K07 M13K07] cloning in ''E. coli'':
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* amino-acid sequence signature (Myc epitope tag) = EQKLISEEDL
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== References ==
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