User:Etienne Robillard/Notebook/Chemtrails911 notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

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Agent Ecoli:Phosphohistidine Transferase Compound

OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters

• Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:

• • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

Experiment

In this experiment the goal is to construct a forensic DNA fingerprinting kit to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).

Experiment checklist

• Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
• DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain.
• familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.
• more checklist fine points here...

See also

• E. coli restriction-modification system
• Synthetic ATP recombinant phosphonates conjugates: phosphoramidate, ANP, cyclic nucleotide phosphodiesterase (CNP), cAMP
• Osmotic regulation of the EnvZ gene : A E. coli switch for sensory autophosphorylation of the histidine kinase ?
• Light-induced drug delivery
• http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG12414&redirect=T
• Beta-galactosidase assay
• EC 2.7.13.3
• Start up genome engineering [with the M13 phage genome] - [a picture is HERE]
  • sequence for M13k07 reengineered plasmid : Note : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting plasmid cloning vector will be a synthetic construct derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce horizontal gene regulation using bacterial conjugation like sex in elephants and the E. coli K-12 MG1655 strain.
• The Matrix (1999)