User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent
Agent Ecoli:Phosphohistidine Transferase Compound
OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters
- Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:
- [Describe here a protocol or method for LuxR/EnvZ dual-input gene knockout (KO) detection and forensic analysis of antiviral phosphoramidate-conjugated compounds with Nitrogen bound molecules known as DNA acid...Include examples for OmpR::Phosphotransferase::Histidine multi-step vector expression in F- repressed colonies in the original/repressed state (control mg 1655 colonies), and finally perform electrophoresis for detection of electrocompetent colonies. In the event of plasmid-free control groups, it is expected that the repression rate is equal to the DNA polymerase transcription rate (but this has not been verified yet...) in luxR repression.]
- Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (18.104.22.168) and this page for references.
In this experiment the goal is to construct a forensic DNA fingerprinting kit to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
- Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
- DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain.
- familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.
- more checklist fine points here...