User:Etienne Robillard/Notebook/Chemtrails911 notebook/Agent Ecoli:PhosphohistidineTransferaseComponent - Revision history

Etienne Robillard: /* Agent Ecoli:Phosphohistidine Transferase Compound */

Etienne Robillard — Wed, 07 Nov 2012 08:52:46 GMT

Agent Ecoli:Phosphohistidine Transferase Compound

←Older revision		Revision as of 08:52, 7 November 2012
Line 1:		Line 1:
	__TOC__		__TOC__
-	== Agent Ecoli:Phosphohistidine Transferase ~~Compound~~ ==	+	== Agent Ecoli:Phosphohistidine Transferase Component ==

	=== OmpR and LuxR ===		=== OmpR and LuxR ===

-	** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.	+	* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
-		+
-	~~=== Experiment ===~~	+
-		+
-	In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).	+
-		+
-	~~==== Experiment checklist ====~~	+
-		+
-	* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.	+
-	* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain.	+
-	* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.	+
-	* more checklist fine points here...	+

	== See also ==		== See also ==

Etienne Robillard: /* OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters */

Etienne Robillard — Wed, 07 Nov 2012 08:52:06 GMT

OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters

←Older revision		Revision as of 08:52, 7 November 2012
Line 2:		Line 2:
	== Agent Ecoli:Phosphohistidine Transferase Compound ==		== Agent Ecoli:Phosphohistidine Transferase Compound ==

-	=== OmpR and LuxR ~~multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters~~ ===	+	=== OmpR and LuxR ===
-		+
-	* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:	+

	** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.		** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.

Etienne Robillard: /* Agent Ecoli:Phosphohistidine Transferase Compound */

Etienne Robillard — Fri, 02 Nov 2012 22:44:44 GMT

Agent Ecoli:Phosphohistidine Transferase Compound

←Older revision		Revision as of 22:44, 2 November 2012
Line 19:		Line 19:
	* more checklist fine points here...		* more checklist fine points here...

-	==== See also ====	+	== See also ==

	* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]		* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]

Etienne Robillard: /* See also */

Etienne Robillard — Fri, 02 Nov 2012 22:44:17 GMT

See also

←Older revision		Revision as of 22:44, 2 November 2012
Line 22:		Line 22:

	* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]		* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
-	* ~~Synthetic ATP recombinant phosphonates conjugates: phosphoramidate, ANP, cyclic nucleotide phosphodiesterase (CNP), cAMP~~	+	* [http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks Standard E. coli Strain for BioBricks] - and this [http://www.freepatentsonline.com/y2003/0138937.html paper]
-	* '''Osmotic regulation of the EnvZ gene :''' A E. coli switch for sensory autophosphorylation of the histidine kinase ?	+
-	* '''Light-induced drug delivery'''	+
-	* http://~~biocyc~~.org/~~ECOLI~~/~~NEW~~-~~IMAGE?type=GENE&object=EG12414&redirect=T~~	+
-	* [http://~~openwetware~~.~~org~~/~~wiki~~/~~Beta-galactosidase_assay Beta-galactosidase assay~~]	+
-	* EC 2.7.13.3	+
	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]		* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
-	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.	+	* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay] - EC 2.7.13.3
		+	* [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''

Etienne Robillard: /* See also */

Etienne Robillard — Fri, 02 Nov 2012 21:46:35 GMT

See also

←Older revision		Revision as of 21:46, 2 November 2012
Line 29:		Line 29:
	* EC 2.7.13.3		* EC 2.7.13.3
	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]		* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
-	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting plasmid will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.	+	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''

Etienne Robillard: /* See also */

Etienne Robillard — Fri, 02 Nov 2012 12:01:54 GMT

See also

←Older revision		Revision as of 12:01, 2 November 2012
Line 29:		Line 29:
	* EC 2.7.13.3		* EC 2.7.13.3
	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]		* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
-	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting plasmid will be a synthetic construct derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.	+	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting plasmid will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''

Etienne Robillard: /* See also */

Etienne Robillard — Fri, 02 Nov 2012 00:04:13 GMT

See also

←Older revision		Revision as of 00:04, 2 November 2012
Line 29:		Line 29:
	* EC 2.7.13.3		* EC 2.7.13.3
	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]		* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
-	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid]	+	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting plasmid will be a synthetic construct derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''

Etienne Robillard: /* See also */

Etienne Robillard — Thu, 01 Nov 2012 23:29:19 GMT

See also

←Older revision		Revision as of 23:29, 1 November 2012
Line 29:		Line 29:
	* EC 2.7.13.3		* EC 2.7.13.3
	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]		* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
-	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid :)]	+	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid]
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''

Etienne Robillard: /* See also */

Etienne Robillard — Thu, 01 Nov 2012 23:27:29 GMT

See also

←Older revision		Revision as of 23:27, 1 November 2012
Line 28:		Line 28:
	* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]		* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]
	* EC 2.7.13.3		* EC 2.7.13.3
-	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]	+	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
		+	** [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid :)]
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''

Etienne Robillard: /* See also */

Etienne Robillard — Thu, 01 Nov 2012 23:11:01 GMT

See also

←Older revision		Revision as of 23:11, 1 November 2012
Line 28:		Line 28:
	* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]		* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]
	* EC 2.7.13.3		* EC 2.7.13.3
-	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg ~~picture~~]	+	* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
	* '''The Matrix (1999)'''		* '''The Matrix (1999)'''