User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

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(OmpR and LuxR multi-step catalytic gene repression systems)
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=== OmpR and LuxR multi-step catalytic gene repression systems ===
=== OmpR and LuxR multi-step catalytic gene repression systems ===
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* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DYI genetic repair:  
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* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection of exogenous drugs or antiviral proteins (GFP/RFP?, etc):
** Describe here a protocol or method for LuxR/EnvZ dual-input gene knockout (KO) detection and forensic analysis of antiviral phosphoramidate-conjugated compounds with Nitrogen bound molecules known as DNA acid...Include examples for OmpR::Phosphotransferase::Histidine multi-step vector expression in F- repressed colonies in the original/repressed state (control mg 1655 colonies), and finally perform electrophoresis for detection of electrocompetent colonies. In the event of plasmid-free control groups, it is expected that the repression rate (Lacl) is equal to the DNA polymerase transcription rate (but this has not been verified yet...).
** Describe here a protocol or method for LuxR/EnvZ dual-input gene knockout (KO) detection and forensic analysis of antiviral phosphoramidate-conjugated compounds with Nitrogen bound molecules known as DNA acid...Include examples for OmpR::Phosphotransferase::Histidine multi-step vector expression in F- repressed colonies in the original/repressed state (control mg 1655 colonies), and finally perform electrophoresis for detection of electrocompetent colonies. In the event of plasmid-free control groups, it is expected that the repression rate (Lacl) is equal to the DNA polymerase transcription rate (but this has not been verified yet...).

Revision as of 21:29, 30 October 2012

Contents

Agent Ecoli:Phosphohistidine Transferase Compound

OmpR and LuxR multi-step catalytic gene repression systems

  • Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection of exogenous drugs or antiviral proteins (GFP/RFP?, etc):
    • Describe here a protocol or method for LuxR/EnvZ dual-input gene knockout (KO) detection and forensic analysis of antiviral phosphoramidate-conjugated compounds with Nitrogen bound molecules known as DNA acid...Include examples for OmpR::Phosphotransferase::Histidine multi-step vector expression in F- repressed colonies in the original/repressed state (control mg 1655 colonies), and finally perform electrophoresis for detection of electrocompetent colonies. In the event of plasmid-free control groups, it is expected that the repression rate (Lacl) is equal to the DNA polymerase transcription rate (but this has not been verified yet...).
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