User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(See also)
(Agent Ecoli:Phosphohistidine Transferase Compound)
(2 intermediate revisions not shown.)
Line 1: Line 1:
__TOC__
__TOC__
-
== Agent Ecoli:Phosphohistidine Transferase Compound ==
+
== Agent Ecoli:Phosphohistidine Transferase Component ==
-
=== OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters ===
+
=== OmpR and LuxR ===
-
* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:
+
* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
-
** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
+
== See also ==
-
 
+
-
=== Experiment ===
+
-
 
+
-
In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
+
-
 
+
-
==== Experiment checklist ====
+
-
 
+
-
* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
+
-
* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain. 
+
-
* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.   
+
-
* more checklist fine points here... 
+
-
 
+
-
==== See also ====
+
* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]

Revision as of 03:52, 7 November 2012

Contents

Agent Ecoli:Phosphohistidine Transferase Component

OmpR and LuxR

  • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

See also

Personal tools