User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

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== Agent Ecoli:Phosphohistidine Transferase Compound ==
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== Agent Ecoli:Phosphohistidine Transferase Component ==
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=== OmpR and LuxR ===
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* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
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=== OmpR and LuxR multi-step catalytic gene repression systems ===
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== See also ==
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* Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DYI genetic repair:  
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* [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system]
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** Describe here a protocol or method for LuxR/EnvZ dual-input gene knockout (KO) detection and forensic analysis of antiviral phosphoramidate-conjugated compounds with Nitrogen bound molecules known as DNA acid...Include examples for OmpR::Phosphotransferase::Histidine multi-step vector expression in F- repressed colonies in the original/repressed state (control mg 1655 colonies), and finally perform electrophoresis for detection of electrocompetent colonies. In the event of plasmid-free control groups, it is expected that the repression rate (Lacl) is equal to the DNA polymerase transcription rate (but this has not been verified yet...).
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* [http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks Standard E. coli Strain for BioBricks] - and this [http://www.freepatentsonline.com/y2003/0138937.html paper]
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* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome]  - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
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* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay] - EC 2.7.13.3
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* [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain.
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* '''The Matrix (1999)'''

Revision as of 03:52, 7 November 2012

Contents

Agent Ecoli:Phosphohistidine Transferase Component

OmpR and LuxR

  • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

See also

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