User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent

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* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]
* [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay]
* EC 2.7.13.3
* EC 2.7.13.3
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* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome]  [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg picture]
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* [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome]  - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]]
* '''The Matrix (1999)'''
* '''The Matrix (1999)'''

Revision as of 19:11, 1 November 2012

Contents

Agent Ecoli:Phosphohistidine Transferase Compound

OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters

  • Reversion and repression of low to medium copy synthetic DNA plasmids (pSB3K3) using proteogenic amino acids and milk as organocatalysts DNA vectors for multi-step, combinatorial gene repression/knockout (KO) recipes for DNA origami forensic detection (N-P bound phosphoramidate), and exogenous drugs or antiviral proteins expressions with high bioluminiscence activity:
    • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

Experiment

In this experiment the goal is to construct a forensic DNA fingerprinting kit to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).

Experiment checklist

  • Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
  • DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain.
  • familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.
  • more checklist fine points here...

See also

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