http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&feed=atom&action=history 2013-05-25T19:50:25Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&diff=663797&oldid=prev 2012-12-14T23:47:23Z

<p><span class="autocomment">Overview</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:47, 14 December 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 23:</td> <td colspan="2" class="diff-lineno">Line 23:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* http://cgsc.biology.yale.edu/Strain.php?ID=11225</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* http://cgsc.biology.yale.edu/Strain.php?ID=11225</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* &gt;= 7.5min = Minimal time needed for TL3' to enter irreversible lytic phase (ie '''prophage lysogenic induction''' state)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* &gt;= 7.5min = Minimal time needed for TL3' to enter irreversible lytic phase (ie '''prophage lysogenic induction''' state)</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* http://openwetware.org/wiki/CH391L/S12/Toggle_Switches,_Repressilators,_and_Counters</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 15:56:25 --> <!-- diff cache key owwdb:diff:version:1.11a:oldid:658884:newid:663797 --> </table>

Etienne Robillard http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&diff=658884&oldid=prev 2012-11-27T01:53:53Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:53, 27 November 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 22:</td> <td colspan="2" class="diff-lineno">Line 22:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* http://cgsc.biology.yale.edu/Strain.php?ID=11225</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* http://cgsc.biology.yale.edu/Strain.php?ID=11225</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* &gt;= 7.5min = Minimal time needed for TL3' to enter irreversible lytic phase (ie '''prophage lysogenic induction''' state)</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 19:50:25 --> </table>

Etienne Robillard http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&diff=658881&oldid=prev 2012-11-27T01:41:40Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:41, 27 November 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 21:</td> <td colspan="2" class="diff-lineno">Line 21:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* piperidine derived protection groups (Tris-HCl, Fmoc, dA, TX100) ??</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* piperidine derived protection groups (Tris-HCl, Fmoc, dA, TX100) ??</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* http://cgsc.biology.yale.edu/Strain.php?ID=11225</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 19:50:25 --> </table>

Etienne Robillard http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&diff=658872&oldid=prev 2012-11-27T01:21:03Z

<p><a href="/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:atc_vs_iptg" title="User:Etienne Robillard/Notebook/Agent Ecoli:atc vs iptg">User:Etienne Robillard/Notebook/Agent Ecoli:atc vs iptg</a> moved to <a href="/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent" title="User:Etienne Robillard/Notebook/Agent Ecoli:ToggleSwitchComponent">User:Etienne Robillard/Notebook/Agent Ecoli:ToggleSwitchComponent</a></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:21, 27 November 2012</td> </tr> <!-- diff generator: internal 2013-05-21 10:31:14 --> <!-- diff cache key owwdb:diff:version:1.11a:oldid:658870:newid:658872 --> </table>

Etienne Robillard http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&diff=658870&oldid=prev 2012-11-27T01:17:41Z

<p><span class="autocomment">Overview</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 01:17, 27 November 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 16:</td> <td colspan="2" class="diff-lineno">Line 16:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* B. subtilis: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC285643/</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* B. subtilis: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC285643/</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* H. influenza: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC246225/</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* H. influenza: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC246225/</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* TL3' = GAT TCT</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* TL3' = GAT TCT &nbsp;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* strong promoter (tetR) repress weak promoter.. (lacZ)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* strong promoter (tetR) repress weak promoter.. (lacZ)</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* PC = PHP ? </ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* piperidine derived protection groups (Tris-HCl, Fmoc, dA, TX100) ??</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 19:50:25 --> </table>

Etienne Robillard http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent&diff=658858&oldid=prev 2012-11-27T00:54:16Z

<p>New page: == atc vs IPTG == === Overview === * tetR (tetracycline resistance gene) + LacR (Lactose resistance gene; LacZ) bistable toggle switch: &quot;TL phage lambda&quot; * Definition of &quot;lysogen&quot; (Wikip...</p> <p><b>New page</b></p><div>== atc vs IPTG ==<br /> <br /> === Overview ===<br /> * tetR (tetracycline resistance gene) + LacR (Lactose resistance gene; LacZ) bistable toggle switch: &quot;TL phage lambda&quot; <br /> * Definition of &quot;lysogen&quot; (Wikipedia): &quot;A lysogen or lysogenic phage is a phage that can exist as a DNA in its dormant state (prophage) within its host organism. A prophage is either integrated into the host bacteria's chromosome or more rarely exists as a stable plasmid within the host cell. The prophage expresses gene(s) that repress the phage's lytic action, until this suppression is disrupted (see lytic cycle). Currently a variety of studies are being conducted to see whether other genes are active during lysogeny, examples of which include phage-encoded tRNA and virulence genes.&quot; [http://en.wikipedia.org/wiki/Lysogen 1]<br /> * IPTG acts as LacR promoter =&gt; prophage positive regulation !<br /> * aTc (tetR): a strong promoter (in contrast to araBAD/pLacZ) !!<br /> * DNA promoter ratio/balance is important key factor to consider !!!<br /> * 5832 combinations possible.. (TL3'?) <br /> * grow lytically in the absence of IPTG...<br /> * http://openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Hrp_System/Parts<br /> * &quot;The original plan for our project was to model the dynamics of the spread of an epidemic within a population. We hoped to make a multi-scale model of epidemic spread and test it in a model population...&quot; <br /> * http://2007.igem.org/Virginia_Tech/Project<br /> * http://rrresearch.fieldofscience.com/2007/05/time-course-and-iptg-dependence.html<br /> * &quot;The results may have implications for competence induction in H. influenzae too.&quot;<br /> * B. subtilis: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC285643/<br /> * H. influenza: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC246225/<br /> * TL3' = GAT TCT<br /> * strong promoter (tetR) repress weak promoter.. (lacZ)</div>

Etienne Robillard