User:Etienne Robillard/Notebook/Agent Ecoli:Strains carrying laclq mutation
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* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid]) | * pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid]) | ||
| - | * | + | * accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]. |
Revision as of 10:30, 5 December 2012
Contents |
Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli
Disclaimer
WOOP WOOP!
Hypothesis
- GCG CAA ? [1]
D1210 perhaps just doesn't fit...
- I need a "non-leaky" strain which express the araBAD mutation.
- lambda lysogen (ie HB101)
- F+ plasmid
- lacY
- hsdR restriction system (ie: leaky restriction system)
- NO appropriate mutation of ARA operon/transport ! WOOP WOOP
BW26434
- Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
- However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
- Are they doing this as a habit or training to become excessively insane?
- A Cloning ninja signature perhaps ?
- Random muslings ?
- ?
- WOOP WOOP !
- pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a cos mid)
- accept inserts up to ~30 to 45 kb 1.
