User:Etienne Robillard/Notebook/Agent Ecoli:Strains carrying laclq mutation
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]. | * accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]. | ||
* WOOP WOOP !!! | * WOOP WOOP !!! | ||
| + | * PHP = amine orthogonal peptide protection group ? (see [http://openwetware.org/wiki/CH391L/S12/Unnatural_Amino_Acids#Solid-phase_Synthesis_and_Chemical_Ligation UAA]) | ||
| + | ** see [[PC]], [[PHP]], [[TE]], [[IPTG]], and [[Benzoyl]] | ||
| + | *** Also see [[Phosphoramidate]] | ||
Current revision
Contents |
Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli
Disclaimer
WOOP WOOP!
Hypothesis
- GCG CAA ? [1]
D1210 perhaps just doesn't fit...
- I need a "non-leaky" strain which express the araBAD mutation.
- lambda lysogen (ie HB101)
- F+ plasmid
- lacY
- hsdR restriction system (ie: leaky restriction system)
- NO appropriate mutation of ARA operon/transport ! WOOP WOOP
BW26434
- Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
- However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
- Are they doing this as a habit or training to become excessively insane?
- A Cloning ninja signature perhaps ?
- Random muslings ?
- ?
- WOOP WOOP !
- pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a cos mid)
- accept inserts up to ~30 to 45 kb 1.
- WOOP WOOP !!!
- PHP = amine orthogonal peptide protection group ? (see UAA)
