User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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=== D1210 perhaps just doesn't fit... ===
=== D1210 perhaps just doesn't fit... ===
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** I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
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* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
** lambda lysogen (ie HB101)
** lambda lysogen (ie HB101)
** F+ plasmid  
** F+ plasmid  
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** ?
** ?
** WOOP WOOP !
** WOOP WOOP !
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* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
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* WOOP WOOP !!!
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* PHP = amine orthogonal peptide protection group ? (see [http://openwetware.org/wiki/CH391L/S12/Unnatural_Amino_Acids#Solid-phase_Synthesis_and_Chemical_Ligation UAA])
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** see [[PC]], [[PHP]], [[TE]], [[IPTG]], and [[Benzoyl]]
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*** Also see [[Phosphoramidate]]

Revision as of 16:49, 5 December 2012

Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • hsdR restriction system (ie: leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

BW26434

  • Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
  • However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
    • Are they doing this as a habit or training to become excessively insane?
    • A Cloning ninja signature perhaps ?
    • Random muslings ?
    •  ?
    • WOOP WOOP !
  • pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a cos mid)
  • accept inserts up to ~30 to 45 kb 1.
  • WOOP WOOP !!!
  • PHP = amine orthogonal peptide protection group ? (see UAA)
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