User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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(BW26434)
Current revision (07:36, 23 August 2015) (view source)
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== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
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{{deleteme}}
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== Disclaimer ==
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WOOP WOOP!
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== Hypothesis ==
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* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
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=== D1210 perhaps just doesn't fit... ===
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* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
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** lambda lysogen (ie HB101)
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** F+ plasmid
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** lacY
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** hsdR restriction system (ie: leaky restriction system)
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** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
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=== BW26434 ===
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* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
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* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
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** Are they doing this as a habit or training to become excessively insane? 
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** A Cloning ninja signature perhaps ?
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** Random muslings ?
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** ?
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** WOOP WOOP !
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* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
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* WOOP WOOP !!!
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Current revision

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Other articles for deletion are listed here.
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