User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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(BW26434)
Current revision (10:42, 31 January 2015) (view source)
 
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== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
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** F+ plasmid  
** F+ plasmid  
** lacY
** lacY
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** hsdR restriction system (ie: leaky restriction system)
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** EGFR-hsdR restriction system (ie: a purely leaky restriction system)
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
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=== BW26434 ===
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=== Biobrick x BW26434 ===
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
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* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
 
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** Are they doing this as a habit or training to become excessively insane? 
 
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** A Cloning ninja signature perhaps ?
 
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** Random muslings ?
 
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** ?
 
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** WOOP WOOP !
 
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]
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* Also see [[Phosphoramidate]].

Current revision

It has been requested that this page be removed with restriction endonucleases.
Other articles for deletion are listed here.


Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • EGFR-hsdR restriction system (ie: a purely leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

Biobrick x BW26434

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