User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo

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Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor

• Date: 2012/12/03 10:29 AM EDT
• Organism: E. coli (K-12 substr.)
• Strain: MG1655(?)

Hypothesis

Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.

Experiment

Material

• 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]

Expected results

• Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
• a heterocyclic base-labile group (NHC?)
• IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)

References

• TE