User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo
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* [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!) | * [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!) | ||
** in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie [http://partsregistry.org/Part:BBa_V1003 laclq]) | ** in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie [http://partsregistry.org/Part:BBa_V1003 laclq]) | ||
| + | ** From [[http://www.sigmaaldrich.com/life-science/molecular-biology/cloning-and-expression/learning-center/genotypes-of-e-coli.html Sigma]], the expected genotype matching the laclq strain is: | ||
| + | *** HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h | ||
==== References ==== | ==== References ==== | ||
Revision as of 15:21, 4 December 2012
Contents |
Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor
- Date: 2012/12/03 10:29 AM EDT
- Organism: E. coli (K-12 substr.)
- Strain: MG1655(?)
Hypothesis
Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
Experiment
Material
- 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
Expected results
- Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
- a heterocyclic base-labile group (NHC?)
- IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
