User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo
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* Date: 2012/12/03 10:29 AM EDT | * Date: 2012/12/03 10:29 AM EDT | ||
* Organism: E. coli (K-12 substr.) | * Organism: E. coli (K-12 substr.) | ||
| - | * Strain: | + | * Strain: [http://cgsc2.biology.yale.edu/Strain.php?ID=65553 BW26434] |
=== Hypothesis === | === Hypothesis === | ||
| - | Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation. | + | * Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation. |
| + | * Cosmid like recombinase system for -35 lacl+ (LacZ) transcription, thus no infection produced; (no lacY) | ||
| + | * orthogonal oligo/peptide protection using methylamino (methyltransferase) agents; | ||
| + | * methylamino buffering agents used as intermediates for orthogonal protection; (probably a side-effect of Magnesium chelation with EDTA?) | ||
| + | * artificial cos-mid size is 30-45 bp length => reduced genome ? | ||
| + | * The NMDA receptor is voltage gated: [http://www.physicsforums.com/showthread.php?t=632781 The addition of EDTA (mg2+) ions to the reaction may act as '''NMDA receptor antagonist''' by binding to the NMDA ligand...] | ||
=== Experiment === | === Experiment === | ||
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* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP | * [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP | ||
* [[IPTG]] | * [[IPTG]] | ||
| + | * [[EDTA]] | ||
Revision as of 10:36, 10 December 2012
Contents |
Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor
- Date: 2012/12/03 10:29 AM EDT
- Organism: E. coli (K-12 substr.)
- Strain: BW26434
Hypothesis
- Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
- Cosmid like recombinase system for -35 lacl+ (LacZ) transcription, thus no infection produced; (no lacY)
- orthogonal oligo/peptide protection using methylamino (methyltransferase) agents;
- methylamino buffering agents used as intermediates for orthogonal protection; (probably a side-effect of Magnesium chelation with EDTA?)
- artificial cos-mid size is 30-45 bp length => reduced genome ?
- The NMDA receptor is voltage gated: The addition of EDTA (mg2+) ions to the reaction may act as NMDA receptor antagonist by binding to the NMDA ligand...
Experiment
Material
- 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
Expected results
- Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
- a heterocyclic base-labile group (NHC?)
- IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
- (2R)-2-(methylamino)succinic acid - An orthogonal N-methyltransferase/NH2 base labile compound; exogenous NMDA receptor antagonist compound (Ketamine-like); synthetically recombined oligonucleotide protection group (dA-dT) for solid-phase peptide synthesis (SPPS), etc (see also PHP, Succinic_acid, and BW26434.)
- important: N-methyl-D-asparte receptor is a "sensory" neurotransmitter with analogous functions to the TRPV neurotransmitter but for brain functions including learning and memory.
