User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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Current revision (17:51, 4 October 2013) (view source)
(BW26434)
 
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** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
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=== BW26434 ===
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=== Biobrick x BW26434 ===
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
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* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
 
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** Are they doing this as a habit or training to become excessively insane? 
 
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** A Cloning ninja signature perhaps ?
 
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** Random muslings ?
 
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** ?
 
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** WOOP WOOP !
 
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
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* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]
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* WOOP WOOP !!!
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* Also see [[Phosphoramidate]].
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* PHP = amine orthogonal peptide protection group ? (see [http://openwetware.org/wiki/CH391L/S12/Unnatural_Amino_Acids#Solid-phase_Synthesis_and_Chemical_Ligation UAA])
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** see [[PC]], [[PHP]], [[TE]], [[IPTG]], and [[Benzoyl]]
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*** Also see [[Phosphoramidate]]
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Current revision

Contents

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • EGFR-hsdR restriction system (ie: a purely leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

Biobrick x BW26434

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