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Revision as of 11:45, 27 November 2012

Agent Ecoli: The whys and wherefores of Angel Dust is in the product.

Overview

• Long-term bioaerosols spraying (ie TL3')
• XXX: may carry a epigenetic trojan horse ?
• TL = toggle bistable switch / circuit

summary

Untested Agent E.coli 1.0 recipe:

• pick at least a combination of 1 (one) nerve agent (ie Benzoyl);
• autoclave custom plasmid(s) with ape... ;
• click on "I UNDERSTAND AND ACKNOWLEDGE THE RISKS OF CLONING FLUORESCENT ELEPHANTS, JUST GIMME SOME HARDCORE PLASMIDS TO MAKE MY CUSTOM OLIGOS!";
• add water (wash in bath with sodium hydrochloride solution..);
• To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);
• To add a good source of aceton to your DNA mix use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);
• For doing oligos version include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as protection group...
• Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphatase regulation.

Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo

Introduction

[TODO]

Chapters

Introduction to Agent E. coli

• Definition of Agent E.coli
  • Basic description
  • Geopolitical background
  • Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
    • It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
  • Classification/Taxonomy
    • Agent E.coli 1.0:
      • Class: Nitrogenous-derived nerve precursor agent. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC).

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

• Synthetic biology and reverse/forward DNA transcription "circuits"
  • luxR: Introduction to the Phosphohistidine transferase genetic switch
  • Agent Ecoli:ToggleSwitchComponent (TL phage lambda)

DNA evidences of synthetic nucleosides analogues are in the products

• dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
• Plasmid addiction systems
  • Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
  • cDNA synthesis (RNAP)

Gibson assembly method

• Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1]
  • [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
  • Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [2]
• See also Gibson Assembly, pSB3K3, BBa_J69512
• In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the pSB3K3 plasmid.

Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties

• Case-study of M13K07 derived genome engineering using site-directed mutagenesis