User:Etienne Robillard/Notebook/Agent Ecoli

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(→summary)
(→Agent Ecoli: The whys and wherefores of Angel Dust is in the product.)
(2 intermediate revisions not shown.)
Line 13: Line 13:
=== summary ===
=== summary ===
-
Untested Agent E.coli 1.0 recipe:
+
Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)
-
* pick at least a combination of 1 (one) nerve agent (ie Benzoyl);
+
== Introduction ==
-
* autoclave custom plasmid(s) with ape... ;
+
-
* click on '''"I UNDERSTAND AND ACKNOWLEDGE THE RISKS OF CLONING FLUORESCENT ELEPHANTS, JUST GIMME SOME HARDCORE PLASMIDS TO MAKE MY CUSTOM OLIGOS!";
+
-
* add water (wash in bath with sodium hydrochloride solution..);
+
-
* To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);
+
-
* To add a good source of aceton to your DNA mix, use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);
+
-
* For doing oligos version, include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as [http://www.organic-chemistry.org/protectivegroups/amino/boc-amino.htm base-labile] protection group...
+
-
* Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphatase regulation.
+
-
Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo
+
* [http://www.openwetware.org/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:Introduction A beginner introduction to Agent E. coli (Start here)]
-
== Introduction ==
 
[TODO]
[TODO]
-
== Chapters ==
+
== Concepts ==
=== Introduction to Agent E. coli ===
=== Introduction to Agent E. coli ===

Revision as of 09:08, 11 January 2013

Agent Ecoli: The whys and wherefores of Angel Dust is in the product.

Contents


Overview

  • Long-term bioaerosols spraying (ie TL3')
  • XXX: may carry a epigenetic trojan horse ?
  • TL = toggle bistable switch / circuit

summary

Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)

Introduction


[TODO]

Concepts

Introduction to Agent E. coli

  • Definition of Agent E.coli
    • Basic description
    • Geopolitical background
    • Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
      • It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
    • Classification/Taxonomy
      • Agent E.coli 1.0:
        • Class: Nitrogenous-derived nerve precursor agent. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC).

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

  • Synthetic biology and reverse/forward DNA transcription "circuits"

DNA evidences of synthetic nucleosides analogues are in the products

  • dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
  • Plasmid addiction systems
    • Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
    • cDNA synthesis (RNAP)

Gibson assembly method

  • Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1]
    • [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
    • Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [2]
  • See also Gibson Assembly, pSB3K3, BBa_J69512
  • In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the pSB3K3 plasmid.

Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties

  • Case-study of M13K07 derived genome engineering using site-directed mutagenesis
Retrieved from "http://openwetware.org/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli"
Personal tools