User:Etienne Robillard/Notebook/Agent Ecoli
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| - | = Agent Ecoli = | + | = Agent Ecoli: The whys and wherefores of Angel Dust is in the product. = |
__TOC__ | __TOC__ | ||
| - | == | + | == Overview == |
| - | Long-term bioaerosols spraying | + | __TOC__ |
| - | + | ||
| + | * Long-term bioaerosols spraying (ie TL3') | ||
| + | * XXX: may carry a '''epigenetic trojan horse''' ? | ||
| + | * TL = toggle bistable switch / circuit | ||
| + | |||
| + | === summary === | ||
| + | |||
| + | Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor) | ||
== Introduction == | == Introduction == | ||
| + | |||
| + | * [http://www.openwetware.org/wiki/User:Etienne_Robillard/Notebook/Agent_Ecoli:Introduction A beginner introduction to Agent E. coli (Start here)] | ||
| + | |||
[TODO] | [TODO] | ||
| Line 14: | Line 24: | ||
== Chapters == | == Chapters == | ||
| - | === Introduction to Agent E. coli | + | === Introduction to Agent E. coli === |
| - | * Definition of Agent E.coli | + | * Definition of Agent E.coli |
| + | ** Basic description | ||
| + | ** Geopolitical background | ||
| + | ** Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ? | ||
| + | *** It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome. | ||
| + | ** Classification/Taxonomy | ||
| + | *** '''Agent E.coli 1.0''': | ||
| + | **** Class: Nitrogenous-derived [http://en.wikipedia.org/wiki/Nerve_agent nerve precursor agent]. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC). | ||
| - | === | + | === Agent E. coli mecanics: Fundamental elements of domestic chemical warfare === |
| - | * Synthetic biology and reverse/forward DNA transcription | + | * Synthetic biology and reverse/forward DNA transcription "circuits" |
| - | ** luxR: Introduction to the Phosphohistidine transferase genetic switch | + | ** luxR: Introduction to the Phosphohistidine transferase genetic switch |
| + | ** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda) | ||
=== DNA evidences of synthetic nucleosides analogues are in the products === | === DNA evidences of synthetic nucleosides analogues are in the products === | ||
| Line 27: | Line 45: | ||
* dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides | * dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides | ||
* Plasmid addiction systems | * Plasmid addiction systems | ||
| - | ** Ape (A Plasmid Editor) | + | ** Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application) |
** cDNA synthesis (RNAP) | ** cDNA synthesis (RNAP) | ||
| + | ==== Gibson assembly method ==== | ||
| + | |||
| + | * Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [[http://www.synbio.org.uk/dna-assembly/guidetogibsonassembly.html 1]] | ||
| + | ** [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.] | ||
| + | ** Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [[http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2]] | ||
| + | * See also [http://openwetware.org/wiki/Haynes:Gibson_Assembly Gibson Assembly], pSB3K3, BBa_J69512 | ||
| + | * In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the '''pSB3K3''' plasmid. | ||
=== Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties === | === Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties === | ||
* Case-study of M13K07 derived genome engineering using site-directed mutagenesis | * Case-study of M13K07 derived genome engineering using site-directed mutagenesis | ||
Revision as of 23:36, 30 November 2012
Agent Ecoli: The whys and wherefores of Angel Dust is in the product.
Overview
- Long-term bioaerosols spraying (ie TL3')
- XXX: may carry a epigenetic trojan horse ?
- TL = toggle bistable switch / circuit
summary
Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo, aspartic acid (NMDA receptor)
Introduction
[TODO]
Chapters
Introduction to Agent E. coli
- Definition of Agent E.coli
- Basic description
- Geopolitical background
- Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
- It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
- Classification/Taxonomy
- Agent E.coli 1.0:
- Class: Nitrogenous-derived nerve precursor agent. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC).
- Agent E.coli 1.0:
Agent E. coli mecanics: Fundamental elements of domestic chemical warfare
- Synthetic biology and reverse/forward DNA transcription "circuits"
- luxR: Introduction to the Phosphohistidine transferase genetic switch
- Agent Ecoli:ToggleSwitchComponent (TL phage lambda)
DNA evidences of synthetic nucleosides analogues are in the products
- dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
- Plasmid addiction systems
- Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
- cDNA synthesis (RNAP)
Gibson assembly method
- Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1]
- [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
- Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [2]
- See also Gibson Assembly, pSB3K3, BBa_J69512
- In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the pSB3K3 plasmid.
Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties
- Case-study of M13K07 derived genome engineering using site-directed mutagenesis
