User:Etienne Robillard/Notebook/Agent Ecoli

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(Gibson assembly)
(Gibson assembly)
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==== Gibson assembly ====
==== Gibson assembly ====
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* [http://openwetware.org/wiki/Haynes:Gibson_Assembly Gibson Assembly]
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* Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [[http://www.synbio.org.uk/dna-assembly/guidetogibsonassembly.html 1]]  
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** "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [[http://www.synbio.org.uk/dna-assembly/guidetogibsonassembly.html 1]] [2009-2010, see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminiscence in dark environments.]
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** [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
** Hopefully we sought not to encounter V. fischeri genes into elephants except perhaps into Cambridge, USA: [[http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2]]
** Hopefully we sought not to encounter V. fischeri genes into elephants except perhaps into Cambridge, USA: [[http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2]]
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* See also [http://openwetware.org/wiki/Haynes:Gibson_Assembly Gibson Assembly]
=== Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties  ===
=== Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties  ===
* Case-study of M13K07 derived genome engineering using site-directed mutagenesis
* Case-study of M13K07 derived genome engineering using site-directed mutagenesis

Revision as of 20:42, 14 November 2012

Agent Ecoli

Contents


Synopsis

Long-term bioaerosols spraying on unwary citizens may act as genetic trojan horse for unethical horizontal gene transfer and recombineering on domestic populations in Quebec.

Introduction

[TODO]

Chapters

Introduction to Agent E. coli and chemical warfare

  • Definition of Agent E.coli
    • Basic description
    • Geopolitical background
  • Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?

It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.

Agent E. coli mecanics: Fundamental elements of domestic chemical warfare

  • Synthetic biology and reverse/forward DNA transcription
    • luxR: Introduction to the Phosphohistidine transferase genetic switch

DNA evidences of synthetic nucleosides analogues are in the products

  • dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
  • Plasmid addiction systems
    • Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
    • cDNA synthesis (RNAP)

Gibson assembly

  • Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [1]
    • [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
    • Hopefully we sought not to encounter V. fischeri genes into elephants except perhaps into Cambridge, USA: [2]
  • See also Gibson Assembly

Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties

  • Case-study of M13K07 derived genome engineering using site-directed mutagenesis
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