User:Etienne Robillard/Notebook/30C6HSL/2013/03/18

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(A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetic mind control)
(A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetic mind control)
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=== A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetic mind control ===
=== A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetic mind control ===
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* 2005 - The search for scFV osmolitic dimer -> ompC dimer protein:  [http://www.uniprot.org/uniprot/P06996.fasta P06996] [[http://openwetware.org/wiki/IGEM:MIT/2005 1]], [][[http://www.ncbi.nlm.nih.gov/protein/56783237 2]] [[http://www.ncbi.nlm.nih.gov/nucleotide/49175990 3]]
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* 2005 - The search for scFV osmolitic dimer -> ompC dimer protein:  [http://www.uniprot.org/uniprot/P06996.fasta P06996] [[http://openwetware.org/wiki/IGEM:MIT/2005 1]], [[http://www.ncbi.nlm.nih.gov/protein/56783237 2]], [[http://www.ncbi.nlm.nih.gov/nucleotide/49175990 3]]
* 2006 - The eau de coli experiment : ''Would E. coli, by any other name, smell as sweet?”''' : http://www.medgadget.com/2006/11/would_e_coli_by_1.html [http://openwetware.org/wiki/IGEM:MIT/2006 1]
* 2006 - The eau de coli experiment : ''Would E. coli, by any other name, smell as sweet?”''' : http://www.medgadget.com/2006/11/would_e_coli_by_1.html [http://openwetware.org/wiki/IGEM:MIT/2006 1]
** leucine auxotrophic gene insert -> osmY http://www.uniprot.org/uniprot/P0AFH8
** leucine auxotrophic gene insert -> osmY http://www.uniprot.org/uniprot/P0AFH8
** ''Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests <font color="red">novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli.''</font> http://www.ncbi.nlm.nih.gov/pubmed/8253679
** ''Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests <font color="red">novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli.''</font> http://www.ncbi.nlm.nih.gov/pubmed/8253679
*** Leucine-responsive regulatory protein (in E.coli K12): See http://www.uniprot.org/uniprot/P0ACJ0
*** Leucine-responsive regulatory protein (in E.coli K12): See http://www.uniprot.org/uniprot/P0ACJ0
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*** http://www.ncbi.nlm.nih.gov/nuccore/D11105 - Link to FASTA sequence file is [[http://www.ncbi.nlm.nih.gov/nuccore/216585?report=fasta|here]].
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*** http://www.ncbi.nlm.nih.gov/nuccore/D11105 - Link to FASTA sequence file is [http://www.ncbi.nlm.nih.gov/nuccore/216585?report=fasta here].
* 2007 - ''Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein.'' http://www.ncbi.nlm.nih.gov/pubmed/14729687?dopt=Abstract and [[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007 1]]
* 2007 - ''Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein.'' http://www.ncbi.nlm.nih.gov/pubmed/14729687?dopt=Abstract and [[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007 1]]
** ''Recovering patients often suffer <font color="red">hallucinations, and sometimes become psychotic</font>'' : http://www.openwetware.org/wiki/Image:IGEM2007_Imperial_iGEMGlobe.png
** ''Recovering patients often suffer <font color="red">hallucinations, and sometimes become psychotic</font>'' : http://www.openwetware.org/wiki/Image:IGEM2007_Imperial_iGEMGlobe.png

Revision as of 16:28, 21 March 2013

Long-term effects of aerosol spraying on E. coli K-12 (MG1655)

Contents


Introduction to CAMK2B protein overexpression

  • examine the acquisition and mutation of the CAMK2B gene in humans subject to horizontal 30C6HSL based transcription promoters (or "devices" for eugenic doctors).
  • Calmodulin dependent inverse/split-pericams and CAMK2B interactions: CAMK2B may depend on exogenous promoters to convey the drug at the right time/place (site-directed mutagenesis). Notice that CAMK2B overexpression is probably an indicator of low neuronal activity (inverse pericams) or osmolitic regulator. (Critical sites: brain (frontal cortex region), amygdala and perhaps the hypothalamus)
  • Never forget atmospheric NH2 as natural source of Nitrogen, hence a ethyl-methyl carbanion may be conjugated to a NH2 molecule in a process known as "benzoin condensation" to produce crystals (N-substituted benzoxazole heterocyclics), thus a form of artificial (asymmetric) Schiff base synthesis may occur as the result of injection of psychochemical intermediates into the atmosphere.

A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetic mind control

Calmodulin kinase type II Beta subunit protein acquisition and expression with 30C6HSL based exogenous promoters (E. coli K-12)

  • GO:1901896 positive regulation of calcium-transporting ATPase activity: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:1901896
  • GO:0005513 detection of calcium ion: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0005513
  • GO:0006970 (osmY) : http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0006970
    • Co-occuring terms/annotations for "osmY" are found here.
  • Significance of CAMK2B gene acquisition and amplification with exogenous 30C6HSL promoters : Within the rigid “β -can” structure (6) of GFP variants, Baird et al. (7) found a site that would tolerate circular permutations where two portions of the polypeptide are flipped around the central site. With obvious clefts in the β-can, the chromophore of the circularly permuted GFPs (cpGFP) seemed to be more accessible to protons outside of the proteins. The use of cpGFP might be interesting for converting the interaction signal between two protein domains into a change in the electrostatic potential of the chromophore; in other words, to transduce the information of the interaction into a fluorescent signal. [1]
  • Browse the annotations for the Yeast (CMK1) ligand here. (EC 2.7.11.17)

Motivations and purposes of psychochemical spraying in the morphogenesis of breast cancer, CES, Parkinson, and Alzheimer diseases via site-directed signaling (mutagenesis)

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