User:Etienne Robillard/Notebook/30C6HSL

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(Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12))
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** google key words: benzodiazepine, oxazole, CAMK2B, S1PR4 (Breast cancer)
** google key words: benzodiazepine, oxazole, CAMK2B, S1PR4 (Breast cancer)
** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977
** Horn-directed "S1PR4" mutant protein amplification and overexpression (Calmodulin/Rhodopsin-dependent orthogonal riboswitch): http://www.uniprot.org/uniprot/O95977
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=== Light (horn) activated S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===
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=== Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12) ===
* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]
* Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with <font color=red>S1PR4-directed</font> protein overexpression lineage in HEK293 important note: <font color="red">''The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16].'' </font> [Note: see [[User:Etienne_Robillard/Notebook/Rhodopsin|Rhodopsin]] for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]
* Questions/Comments:
* Questions/Comments:

Revision as of 20:57, 29 March 2013

Contents

30C6HSL: Adventures in the land of light-activated psychochemical agents

N-substituted arylcyclohexylamines (3-oxo-hexanoyl-homoserine lactone)

  • Chuck: Are you sure this is it this time?
  • Oracle: Trust me.
  • Oracle: 30C6HSL is really your thing.
  • Chuck: Fine. I imagine you want to make a point about LuxR.
  • Oracle: I beg to differ ?
  • Chuck: Dude, you are hard to follow sometimes.
  • Oracle: I suggest you pass more time studying how C6 homoserine lactones enter nitrogen and purine metabolism.
  • Chuck: I have impression C6 hexanoyl may hold the clue and condense to form the 1,2,3,4-tetrahydroisoquinoline enantiomer.
  • Oracle: Shh. You have some balls to put this there without any warning shot.
  • Chuck: I beg to differ...
  • Chuck: I have no mean to accept psychochemical warfare without any warning shot.
  • Oracle: Right. You kids dont understand well the psychology of war.
  • Chuck: My fb account is loaded with slave cuties.
  • Oracle: LOL

BBa_C0061: visualizing LuxR/EnvZ pathways through 30C6HSL

'Yeast based quorum sensing and trans-splicing evidences in H. sapiens: Transforming E. coli with AHLs - BBa_C0061

  • ATCC 96604 (P48445) - Download: FASTA FILE 1
  • ATCC 204508/S288c - UNIPROT (Adenylyl cyclase-associated protein) : P17555

Design of your mutant g-protein receptor: From 30C6HSL to Heterocyclics oxazole

In regard of fine tuning E. coli for mutant protein acquisition, amplification, and overpression of the CAMK2B gene, the following excerpt from the OMIM.org database has this to say on the relevance of CAMK2 osmolitic dimer: "By Western blot analysis, Thiagarajan et al. (2002) found that the protein levels of Camk2a and Camk2b were inversely related during activity in cultured rat pup hippocampal neurons. The levels of Camk2a increased with heightened activity, and the levels of Camk2b increased with suppression of activity. Thiagarajan et al. (2002) hypothesized that the change in the ratio may help tune the Camk2 holoenzyme to changing intensities of Ca(2+) signaling." 1

Light (round the horn) directed S1PR4 mutant protein amplification and overexpression in HEK293 cells lineage (E. coli K12)

  • Breast cancer/Alzheimer/Leukemia (DS, Parkinson, etc): Rhodopsin (Rho) gene-level interactions with S1PR4-directed protein overexpression lineage in HEK293 important note: The Rho signalling pathway is activated, for example, by bioactive sphingolipids such as sphingosine-1-phosphate (SPP) or by overexpression of Rho family members in tumorigenesis and metastases [16]. [Note: see Rhodopsin for info on photophosphorylation of the Rhodopsin kinase, a Ca2-dependent photoreceptor/GPCR]
  • Questions/Comments:
  1. Which light-activated (AHL) synthetic transcription promoters the DNA engineers may have chosen to induce Calmodulin-dependent psychotic behavior and visual hallucinations as the primary phenotype? -ER
  2. What is the technique (protocol name) name the "biological programmers" may have implemented to induce synthetic neurological circuits or programs? -ER
  3. Based on your research, what is a neuronal aptamer primary biological functions and describe the key concepts and techniques in the "hijacking" of human DNA as a information storage transcriptional vector -ER
  • Notes:
  1. To obtain the consensus pattern, see Accession number PS00237 here. (FASTA).
  2. Ligands/motifs bindings: http://www.ebi.ac.uk/pdbe-site/pdbemotif/barChartPattern?pattern1=PS50262
  3. Uniprot dataset (S1PR4 g-receptor): http://www.uniprot.org/uniprot/O95977

References

  1. Wagner S, Klepsch MM, Schlegel S, Appel A, Draheim R, Tarry M, Högbom M, van Wijk KJ, Slotboom DJ, Persson JO, and de Gier JW. . pmid:18796603. PubMed HubMed [ref1]
  2. Nagai T, Sawano A, Park ES, and Miyawaki A. . pmid:11248055. PubMed HubMed [ref2]
All Medline abstracts: PubMed HubMed
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