User:Emmalee Jones/Notebook/Lab Notebook/2010/04/23: Difference between revisions
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== | ==Flow Cells== | ||
* Made up a flow cell with MTs and lipids | * Made up a flow cell with MTs and lipids | ||
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* Quoting from the Dairy Science paper: "Proteins that contain negatively charged groups, such as carboxylate or phosphoserine groups, will bind to the poly-L-lysine, and the complete surface of the grid becomes covered with immobilized protein." | * Quoting from the Dairy Science paper: "Proteins that contain negatively charged groups, such as carboxylate or phosphoserine groups, will bind to the poly-L-lysine, and the complete surface of the grid becomes covered with immobilized protein." | ||
* This paper talks about interaction of Egg PC (70% of the lipids in the vesicles) and casein: Fang, Dalgleish - Studies on Interactions between Phosphatidylcholine and Casein - Langmuir - 1995. Casein does not seem to interact with vesicles once they are made, but it can be incorporated into vesicles. | |||
* This article talks more about lipid and casein interaction: Membrane interactions and lipid binding of casein oligomers and early aggregates, Sokolovski, Biochimica et Biophysica Acta, 2008. Basically, lipids bind to casein more when they have a charge. | |||
* I need to look at the container of Texas Red in the CBME, but for now I think I found the general type that it is. I'll look up the specifics later. This is the link for one of the versions from Sigma: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=S3388|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC | |||
Revision as of 17:24, 23 April 2010
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Flow Cells
1) Wash flow cell with casein/pem
Interaction of lysine with TR, casein, BSA My vials of casein/pem have a green line through the label Order more lysine - DONE - delivering to CBME Could also look at BSA instead of casein
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