User:Elizabeth Schott/Notebook/Nanoparticles/2015/06/02: Difference between revisions
(Autocreate 2015/06/02 Entry for User:Elizabeth_Schott/Notebook/Nanoparticles) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==June 02 2015: Synthesizing Au Nanoparticles Pt 5== | ||
Stock Solution: Sodium Citrate (Na2C4H5O7 * 2H2O) | |||
A 1%(w/v) stock solution of sodium citrate was prepared for this experiment. 1.053 g of sodium citrate was weighed in a plastic wayboat on a scale and transferred into a 50 mL beaker. The wayboat was rinsed with water and emptied into the 50 mL beaker to ensure maximum sodium citrate transfer. Water was added to the 50 mL beaker and was stirred to dilute the solvent. The solution was then transferred into a 100 mL volumetric flask. The remaining space in the flask was filled with water and stirred. This solution was emptied from the volumetric flask into a sealed container and labeled. | |||
Stock Solution: Gold Solution (HAuCl4) | |||
This .001 mM stock solution was modified to use HAuCl4 in place of HAuClr*3H2O (see calculations 1). 0.17 g of HAuCl4 was weighed in a plastic wayboat on a scale and transferred into a 50 mL beaker. The wayboat was rinsed with water and emptied into the 50 mL beaked to ensure maximum transfer. Water was added to the 50 mL beaker and was stirred to dilute the solvent. The solution was then transferred into a 500 mL volumetric flask. The remaining space in the flask was filled with water and stirred. This solution was emptied from the volumetric flask into a sealed container and labeled. | |||
Procedure | |||
1) A hot plate was obtained and placed under the fume hood, with heat set to level 4 and stirring set to level 2. | |||
2) A 50 mL beaker and stirring rod were obtained. After placing stirring rod in beaker, 22 mL of water was measured using a graduated cylinder and transferred into the beaker. The water line was then marked, and the water was disposed. Using a graduated cylinder, 20 mL of the stock gold solution was measured and transferred into the beaker containing the stirring rod. This beaker was then placed on the hot plate in the fume hood and a timer was set. | |||
3) When the solution in the beaker began to boil, the time since placement was recorded. Using a P1000 micro-pipette, 2 mL of the stock sodium citrate solution was added into the beaker. The time of color change from light yellow to purple was then recorded. | |||
4) Water was added to the solution using a transfer pipette to keep water line at marked level. | |||
5) Solution was left to change into a dark red color, which it was then removed and the time recorded. | |||
''Results'' | |||
[[Image:602pic1.png|300px]] | |||
[[Image:602pic2.png|500px]] | |||
[[Image:602pic3.png|500px]] | |||
The first table shows the time tables at which there was an observed color change and when solution was removed from heat. Table 2 shows initial data calculations. Table 3 shows baselined data calculations. | |||
''Discussion'' | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 11:31, 2 June 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
June 02 2015: Synthesizing Au Nanoparticles Pt 5Stock Solution: Sodium Citrate (Na2C4H5O7 * 2H2O) A 1%(w/v) stock solution of sodium citrate was prepared for this experiment. 1.053 g of sodium citrate was weighed in a plastic wayboat on a scale and transferred into a 50 mL beaker. The wayboat was rinsed with water and emptied into the 50 mL beaker to ensure maximum sodium citrate transfer. Water was added to the 50 mL beaker and was stirred to dilute the solvent. The solution was then transferred into a 100 mL volumetric flask. The remaining space in the flask was filled with water and stirred. This solution was emptied from the volumetric flask into a sealed container and labeled. Stock Solution: Gold Solution (HAuCl4) This .001 mM stock solution was modified to use HAuCl4 in place of HAuClr*3H2O (see calculations 1). 0.17 g of HAuCl4 was weighed in a plastic wayboat on a scale and transferred into a 50 mL beaker. The wayboat was rinsed with water and emptied into the 50 mL beaked to ensure maximum transfer. Water was added to the 50 mL beaker and was stirred to dilute the solvent. The solution was then transferred into a 500 mL volumetric flask. The remaining space in the flask was filled with water and stirred. This solution was emptied from the volumetric flask into a sealed container and labeled. Procedure 1) A hot plate was obtained and placed under the fume hood, with heat set to level 4 and stirring set to level 2. 2) A 50 mL beaker and stirring rod were obtained. After placing stirring rod in beaker, 22 mL of water was measured using a graduated cylinder and transferred into the beaker. The water line was then marked, and the water was disposed. Using a graduated cylinder, 20 mL of the stock gold solution was measured and transferred into the beaker containing the stirring rod. This beaker was then placed on the hot plate in the fume hood and a timer was set. 3) When the solution in the beaker began to boil, the time since placement was recorded. Using a P1000 micro-pipette, 2 mL of the stock sodium citrate solution was added into the beaker. The time of color change from light yellow to purple was then recorded. 4) Water was added to the solution using a transfer pipette to keep water line at marked level. 5) Solution was left to change into a dark red color, which it was then removed and the time recorded.
The first table shows the time tables at which there was an observed color change and when solution was removed from heat. Table 2 shows initial data calculations. Table 3 shows baselined data calculations.
|