User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/27: Difference between revisions
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==Description== | ==Description== | ||
* The hydrogels were removed from the freezer at 10 am and were put back in at 2 pm. | |||
* The quaternary ammonium salt films made on monday could not be crosslinked because they had not set. | |||
* The 10% QAS film was filtered using a vacuum pump and a buchner funnel. | |||
<u> | |||
<u>Quaternary Ammonium Salt Film Preparation</u> | |||
# Measure out two 1 g samples of the 1% QAS | |||
# Place in beaker with 30 mL water. Dissolve on heat. | |||
# Once dissolved, add 1 mL glutaraldehyde. Allow to stir for 3 minutes. | |||
# Pour into plastic dish and allow to evaporate until Friday. | |||
<u>Next Hydrogel Reaction (Click Chemistry)</u> | |||
* PNAS article <u>A chemical method for fast and sensitive detection of DNA synthesis in vivo</u> by Adrian Salic and Timothy J. Mitchison was used to determine the ranges of concentrations for the reactants for the click reaction. | |||
* The article uses 0.5 - 1 mM CuSO<sub>4</sub>, 1 - 100 μM fluorescent azide (in 10 - 100 mM DMSO stock), and 50 - 100 mM ascorbic acid (added last from a stock solution of 0.5 M) | |||
* They reacted for 30 minutes at room temperature | |||
* The dye should be in a solution of DMSO | |||
* We will add 1.5X excess of dye | |||
* Will use the same concentration of CuSO<sub>4</sub> and ascorbic acid as papers (so depends on however much water we decide to add) | |||
==Data== | ==Data== | ||
No data was collected today. | |||
==Notes== | ==Notes== | ||
* Next step is to figure out the amount of alkyne (assuming no loss) and then calculate the rest of the concentrations | |||
Revision as of 08:26, 3 April 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTake out the hydrogels to thaw, crosslink the quaternary ammonium salt films made monday (0.01%), make 1% QAS film, filter the 10% QAS, and look up concentrations for the next hydrogel reaction. Description
DataNo data was collected today. Notes
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