User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04: Difference between revisions
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==Description== | ==Description== | ||
<u>Starter Culture Preparation</u> | |||
# Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes | |||
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | |||
# Place the tubes on a shaker at 37°C overnight. | |||
<u>Agar Plate Preparation</u> | <u>Agar Plate Preparation</u> | ||
# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | # Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | ||
# The | # The solution was autoclaved for one hour | ||
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | # Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | ||
#* This made 20 agar plates | #* This made about 20 agar plates | ||
# The plates were left in the fridge to set | |||
==Data== | ==Data== | ||
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==Notes== | ==Notes== | ||
* Because of snow day this week, bacteria were left on shaker until Thursday. | |||
<u>To Do List this Week</u> | <u>To Do List this Week</u> |
Revision as of 19:13, 26 March 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo prepare agar plates and to prepare the starter culture of bacteria. DescriptionStarter Culture Preparation
Agar Plate Preparation
DataNo data was collected today Notes
To Do List this Week
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