User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/10/03: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
(One intermediate revision by the same user not shown) | |||
Line 11: | Line 11: | ||
==Objective== | ==Objective== | ||
To crosslink the two clay films, make a 1 gram PVOH film with glutaraldehyde, and | To crosslink the two clay films, make a 1 gram PVOH film with glutaraldehyde, and dilutions of the filtrates from the clay-copper sulfate exchanges | ||
==Description== | ==Description== | ||
Line 17: | Line 18: | ||
<u>Crosslinking Clay Films</u> | <u>Crosslinking Clay Films</u> | ||
# | # Allow the PVOH film containing glutaraldehyde to dry completely. | ||
# | # Prepare a solution of 100 mL H<sub>2</sub>O, 2 mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20 g Na<sub>2</sub>SO<sub>4</sub> in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | ||
# | # Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | ||
# Heat solution to 70°C (temperature on hotplate should be about 160) | |||
# Place film in solution for one hour. | |||
# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
# Heat HCl solution to 70°C | |||
# Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
# Place film into HCl solution for 30 minutes. | |||
# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
# Use tweezers to remove the film from the sodium bicarbonate solution. | |||
# Rinse film with deionized water and dry film on paper towel. | |||
<u>Making a 1g Film</u> | |||
# 0.5 g of PVOH was dissolved in 5 mL of water and was placed on a hotplate | |||
# 1 mL of glutaraldehyde was added to the solution under the hood. | |||
# The mixture was allowed to stir for about 3 minutes. | |||
# The film was poured into a dish and was left under the hood over the weekend | |||
Revision as of 21:37, 25 November 2012
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo crosslink the two clay films, make a 1 gram PVOH film with glutaraldehyde, and dilutions of the filtrates from the clay-copper sulfate exchanges
DescriptionCrosslinking Clay Films
Data
NotesThis area is for any observations or conclusions that you would like to note.
|