User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/04/04
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo determine the number of lysine residues that bound to the dye molecules. Description1. Determine the concentration of a known dye solution. The dye in solution has been prepared on the 21/2 using: m = 1mg of dye 5ml of water + 80µL of DMSO => Total volume = V = 0.0058L MW of the dye = 573.51 g/mol So, n = 10^-3/573.51 = 1.74* 10^-6 moles And, C = (1.74* 10^-6)/0.0058 = 3*10^-4 M 2. Determine the molar absorptivity of the dye at 602 nm using a dye solution with a known concentration by using Beer's Law. Molar absorbtivities (ε, L/mol/cm) were determined using Beer's law: A = ε*l*C With A = absorbance at 602nm l = width of the cuvette (1cm) C = dye concentration (3*10^-4 M) The molar absorptivity of our dye at 602nm is equal to = 3419 L.mol^-1.cm^-1 (It's determined by doing the average of the three different ε values) 3. Determine the molar absoptivity of the dye at 625 nm using a dye solution with a known concentration by using Beer's Law Molar absorbtivities (ε, L/mol/cm) were determined using Beer's law: A = ε*l*C With A = absorbance at 625nm l = width of the cuvette (1cm) C = 3*10^-4 M The molar absorptivity of our dye at 625nm is equal to = 2204 L.mol^-1.cm^-1 (It's determined by doing the average of the three different ε values) 4. Determine the dye concentrations of the 70 molar ratio, 166 molar ratio and the BSA/HCl dye reactions In this table are corrected value for Absorbance (Abs with dye - Abs without dye) According to these data, we can calculate the dye concentration using Beer's law. We know that: ε at 625nm = 2204 L.mol^-1.cm^-1 ε at 602nm = 3419 L.mol^-1.cm^-1 Data
NotesThis area is for any observations or conclusions that you would like to note.
|