User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/04/04: Difference between revisions
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==Objective== | ==Objective== | ||
To | To determine the number of lysine residues that bound to the dye molecules. | ||
==Description== | ==Description== | ||
1. Determine the concentration of a known dye solution. | |||
The dye in solution has been prepared on the 21/2 using: | |||
m = 1mg of dye | |||
5ml of water + 80µL of DMSO => Total volume = V = 0.0058L | |||
MW of the dye = 573.51 g/mol | |||
So, n = 10^-3/573.51 = 1.74* 10^-6 moles | |||
And, C = (1.74* 10^-6)/0.0058 = 3*10^-4 M | |||
2. Determine the molar absorptivity of the dye at 602 nm using a dye solution with a known concentration by using Beer's Law. | |||
==Data== | ==Data== | ||
Revision as of 20:16, 23 April 2012
 Experimental Biological Chemistry
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ObjectiveTo determine the number of lysine residues that bound to the dye molecules. Description1. Determine the concentration of a known dye solution. The dye in solution has been prepared on the 21/2 using: m = 1mg of dye 5ml of water + 80µL of DMSO => Total volume = V = 0.0058L MW of the dye = 573.51 g/mol So, n = 10^-3/573.51 = 1.74* 10^-6 moles And, C = (1.74* 10^-6)/0.0058 = 3*10^-4 M 2. Determine the molar absorptivity of the dye at 602 nm using a dye solution with a known concentration by using Beer's Law. Data
NotesThis area is for any observations or conclusions that you would like to note.
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