User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/02/15: Difference between revisions

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==Description==
==Description==
<u>Lowering the pH</u>
<u>Lowering the pH</u>
1. BSA Control Solution
1. BSA Control Solution
* 16.8 μL if 0.948 M HCl was added and the pH dropped to 3.20 and the solution became pink
* 16.8 μL if 0.948 M HCl was added and the pH dropped to 3.20 and the solution became pink

Revision as of 11:05, 21 February 2012

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Objective

To lower the pH of the reaction mixtures to approximately 7.00 and to perform dialysis on the samples.

Description

Lowering the pH

1. BSA Control Solution

  • 16.8 μL if 0.948 M HCl was added and the pH dropped to 3.20 and the solution became pink
  • Added 300 μL 50 m Tris and the pH rose to 6.49 and the solution became more purple

2. Fiber Solution (166 ratio)

  • Added 79μL if 0.948 M HCl, pH dropped to 2.72
  • Added 1 mL of Tris, pH rose to 6.14


Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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