User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/26: Difference between revisions
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==Objective== | ==Objective== | ||
To destain the protein gel prepared the previous day and to synthesize gold nanoparticles. | |||
==Description== | ==Description== | ||
# Two gold nanoparticle synthesis reactions were prepared. Tube 1 contained 1 mL of BSA and 9 mL of H<sub>2</sub>O. Tube 2 contained 1 mL of BSA, 2 mL of HAuCl<sub>4</sub>, and 7 mL of water. | |||
# Both tubes were placed in an oven set to 70°C for 2.5 hours. Every 30 minutes, the tubes were removed from the oven for 10 minutes. | |||
==Data== | ==Data== | ||
t = 30 mins - Tubes A and B had bubbles forming in the solution. Tube B had more bubbles than tube A. | |||
t = 60 mins - Tube A remained unchanged, while Tube B had dark particles beginning to form. | |||
t = 90 mins - Tube A was clear and Tube B remained unchanged. | |||
t = 120 mins - Tube A was clear and Tube B remained unchanged. | |||
t = 150 mins - Tube A was clear and Tube B remained unchanged. | |||
==Notes== | ==Notes== |
Revision as of 11:33, 8 November 2011
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ObjectiveTo destain the protein gel prepared the previous day and to synthesize gold nanoparticles. Description
Datat = 30 mins - Tubes A and B had bubbles forming in the solution. Tube B had more bubbles than tube A. t = 60 mins - Tube A remained unchanged, while Tube B had dark particles beginning to form. t = 90 mins - Tube A was clear and Tube B remained unchanged. t = 120 mins - Tube A was clear and Tube B remained unchanged. t = 150 mins - Tube A was clear and Tube B remained unchanged. NotesThis area is for any observations or conclusions that you would like to note.
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